Abcam, ab32127, Anti-Synaptophysin antibody [YE269] - Synaptic Marker

Size: 100µL / 1mL
Anti-Synaptophysin antibody [YE269] (ab32127) is a rabbit monoclonal antibody detecting Synaptophysin in Western Blot, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 310 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:YE269,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-Synaptophysin antibody [YE269] (ab32127) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P and WB.
Anti-Synaptophysin antibody [YE269] (ab32127) was first used in a scientific publication in 1986 and has been cited over 311 times in peer reviewed journals. It's performance in Western blot and IHC in mouse samplesis trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Synaptophysin antibody [YE269] (ab32127) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-Synaptophysin antibody [YE269] (ab32127) has been confirmed by Western Blot testing in Synaptophysin knockout HEK-293T cells (
ab255356
Anti-Synaptophysin antibody [YE269] (ab32127) has 38 independent reviews from customers.
Anti-Synaptophysin antibody [YE269] (ab32127) specifically detects Synaptophysin (UniProt ID: P08247; Molecular weight: 34kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone YE269 -
ab187259
Antibody clone YE269 is also available pre-conjugated to a variety of labels for your convenience - HRP, Alexa Fluor
647, Alexa Fluor
488, Alexa Fluor
594, Alexa Fluor
555, (
ab195520
ab196166
ab196379
ab206868
ab206870
Synaptophysin (SYP) is a key protein in neuro research, particularly for its role as a marker of synaptic vesicles. It is involved in synaptic transmission and vesicle trafficking. Researchers use synaptophysin to study synaptic density and integrity, making it essential for understanding synaptic function and dysfunction in various neurological conditions, including Alzheimer's disease (AD) and schizophrenia. Its expression helps in assessing synaptic health and the effects of neurodegenerative diseases on synaptic connectivity.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Synaptophysin also known as SYP is a glycoprotein approximately 38 kDa in size. It is expressed abundantly in the presynaptic vesicle membranes of neurons and in neuroendocrine cells. Synaptophysin serves as a marker for synaptic vesicles and neuroendocrine tumors. The protein plays a significant role in synaptic vesicle cycling processes engaging in the release and recycling of neurotransmitters.
Biological function summary
Synaptophysin contributes to synaptic transmission and is engaged in the formation and maintenance of the synaptic vesicle pools. It is a part of the complex involved in neuronal communication and might interact with other vesicular proteins to ensure efficient neurotransmitter exchange. Researchers often utilize anti-Synaptophysin antibodies such as Alexa Fluor 647 labeled ones for visualization under confocal microscopy.
Pathways
Synaptophysin operates within pathways involving neurotransmitter release and synaptic vesicle cycle. It connects to proteins like Synaptotagmin and SNAP-25 integral to the facilitation of synaptic transmission and exocytosis. These pathways ensure efficient signal conduction across neurons which is critical for normal nervous system functioning.
Synaptophysin shows a strong association with neurodegenerative diseases and certain cancers like neuroblastoma. Altered expression levels or dysfunction of Synaptophysin can serve as a diagnostic marker for these conditions. The protein is connected with proteins like Bcl-2 in neurodegenerative diseases affecting cell survival and apoptosis pathways.