Abcam, ab32129, Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade

Size: 20µL / 100µL / 1mL
Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129) is a rabbit monoclonal antibody detecting Histone H3 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF, ChIP, PepArr . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 40 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:Y28,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, ChIP, CUT&Tag, PepArr, Flow Cyt (Intra), WB, ICC/IF, IP, ChIP-seq, ChIC/CUT&RUN-seqSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody has weak cross-reactivity with H3R8me1 and H3K4me3 in peptide array. Further optimization may be needed to minimize the cross-reactivity in assays similar to peptide array

Product details:
What is this antibody validated in?
Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP, Peptide Array in Human, Mouse, Rat samples.
What is the molecular weight of Histone H3?
Anti-Histone H3 (acetyl K9) [Y28] - ChIP Grade (ab32129) specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15kDa.
Trusted by the scientific community
Anti-Histone H3 (acetyl K9) [Y28] - ChIP Grade (ab32129) was first used in a scientific publication in 2006 and has been cited over 40 times in peer-reviewed journals.
Reviewed by scientists
Anti-Histone H3 (acetyl K9) [Y28] - ChIP Grade (ab32129) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [Y28] also available for your convenience: ab32129, Alexa Fluor® 488 -
ab200986
, Alexa Fluor® 647 -
ab200989
, Carrier free -
ab203951
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle