Abcam, ab321809, Anti-ATP6V0D2 antibody [EPR29173-521]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal ATP6V0D2 antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Human, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR29173-521,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP6V0D2 also known as V-type proton ATPase subunit d 2 is a subunit of the V-ATPase complex. It has a mass of approximately 38 kDa. This protein is expressed in various tissues with significant expression noted in bone and white adipose tissue. Mechanically ATP6V0D2 contributes to the acidification of intracellular compartments. It functions as part of the proton pump machinery facilitating the transfer of protons across membranes which is essential for processes like endocytosis and intracellular trafficking.
Biological function summary
ATP6V0D2 plays a significant role in bone resorption and adipogenesis. It is a component of the larger V-ATPase complex important for creating acidic environments required for enzyme activity particularly in osteoclasts. This acidity enables the breakdown of bone matrix which is a fundamental part of bone remodeling. Additionally ATP6V0D2 influences fat cell differentiation by modulating the function of cytosolic components within the complex.
Pathways
ATP6V0D2 functions as an integral part of the bone remodeling and lysosomal degradation pathways. In the bone remodeling pathway the protein works closely with other proton pump components like ATP6V0A3 to regulate osteoclast activity. In lysosomal degradation it aids in maintaining the acidity essential for lysosomal enzyme activity. Both pathways are connected through ATP6V0D2's regulation of intracellular pH and ion homeostasis.
ATP6V0D2 has associations with osteoporosis and obesity. In osteoporosis deficient or dysfunctional ATP6V0D2 activity can impair osteoclast function leading to altered bone resorption and skeletal fragility. In the context of obesity this protein influences adipocyte differentiation and function. ATP6V0D2's role in these disorders connects it to other proteins like RANKL in bone metabolism and PPARγ in adipogenesis linking it beyond just its structural function within proton pumps.