Abcam, ab32328, Anti-RhoGAP antibody [EP489Y]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal RhoGAP antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 4 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP489Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RhoGAPs also known as Rho GTPase-activating proteins perform a critical mechanical role by converting active GTP-bound Rho GTPases into their inactive GDP-bound form. RhoGAPs regulate this GTPase activity through their GAP domain acting as molecular switches in various signaling pathways. The typical mass of RhoGAPs can vary around 50-150 kDa depending on the specific family member. Expression of RhoGAPs occurs ubiquitously across many cell types reflecting their importance in cellular signaling processes.
Biological function summary
RhoGAPs regulate the actin cytoskeleton influencing cell mobility shape and cycle progression. These proteins often work as part of a larger complex interacting with other signaling molecules to modulate cytoskeletal dynamics. By keeping Rho GTPases activities in check RhoGAPs play essential roles in maintaining normal cellular function and dynamics. They contribute to processes such as cell migration proliferation and differentiation.
Pathways
RhoGAPs integrate into the Rho/Rac/Cdc42 signaling pathways serving as key regulators of actin cytoskeleton reorganization. In these pathways RhoGAPs balance the activity of Rho GTPases to ensure proper response to stimuli. Together with proteins such as RhoGEFs which activate Rho GTPases RhoGAPs form a regulatory network that precisely controls cytoskeletal and membrane dynamics critical for effective cellular responses.
Defects in RhoGAP function have been implicated in cancer progression and neurodegenerative disorders. Dysfunctional RhoGAP activity can lead to unregulated cell proliferation and survival contributing to cancer development. Similarly in neurological contexts aberrant RhoGAP signaling can affect neuron structure and function potentially linking to Alzheimer's disease. These associations highlight the importance of RhoGAPs and their interactions with Rho GTPases in maintaining cellular homeostasis and human health.