Abcam, ab32425, Anti-CBL + CBLB antibody [YE315]

Size: 100µL
Rabbit Monoclonal CBL antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:YE315,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The immunogen sequence is 100% identical for both CBL and CBLB, therefore there is likely to be cross-reactivity.

Product details:
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
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contact us
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Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target proteins CBL (Casitas B-lineage lymphoma) and CBLB (CBL proto-oncogene B) function as E3 ubiquitin ligases regulating protein ubiquitination and degradation. These proteins are also known by other names including CBL and CBL-B. CBL has a molecular mass of around 120 kDa while CBLB is about 982 amino acids in size. Researchers find their expression mainly in hematopoietic cells though they are also present in other cell types. CBL and CBLB regulate signaling pathways that are key to cellular homeostasis and function.
Biological function summary
CBL and CBLB play roles in the negative regulation of cellular signaling cascades. These proteins are key players in the regulation of receptor tyrosine kinases (RTKs) by marking them for degradation through ubiquitination. This process places CBL and CBLB in a complex with signaling molecules where they help to modulate signal transduction processes. These activities are essential for maintaining normal cellular responses and preventing aberrant signaling.
Pathways
CBL and CBLB integrate with multiple signaling pathways notably the EGFR and T-cell receptor pathways. They interact with proteins such as growth factor receptor-bound protein 2 (GRB2) and phospholipase C gamma-1 (PLCγ1) to control signal propagation and termination. Their roles ensure that the pathways remain balanced therefore preventing excessive or insufficient signaling which could lead to pathophysiological conditions.
Mutations or dysfunction in CBL and CBLB associate with several conditions such as Noonan syndrome and certain cancers. CBL mutations have links to the development of various leukemias while abnormalities in CBLB may contribute to autoimmune disorders. Through these conditions they connect with proteins like SHP-2 which is similarly implicated in Noonan syndrome highlighting their significance in disease mechanisms.