Abcam, ab32441, Anti-SIRT1 antibody [E104]

Size: 20µL / 100µL / 1mL
Anti-SIRT1 antibody [E104] (ab32441) is a rabbit monoclonal antibody detecting SIRT1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 150 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:E104,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), IP, IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody does not cross-react with other sirtuin family members. Expression levels of the target protein vary with sample type and some optimisation may be required. For western blotting, more concentrated lysates may be required when using tissues samples.

Product details:
What is this antibody validated in?
Anti-SIRT1 antibody [E104] (ab32441) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of SIRT1?
Anti-SIRT1 [E104] (ab32441) specifically detects a band for SIRT1 (UniProt: Q96EB6) at a molecular weight of 82kDa.
Trusted by the scientific community
Anti-SIRT1 [E104] (ab32441) was first used in a scientific publication in 2006 and has been cited over 150 times in peer-reviewed journals.
Reviewed by scientists
Anti-SIRT1 [E104] (ab32441) has over 10 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
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Specificity confirmed
The specificity of Anti-SIRT1 antibody [E104] (ab32441) has been confirmed by Western blot testing in SIRT1 Knockout HEK-293 cell line.
Other related products
We have a range of other formats of antibody clone [E104] also available for your convenience: ab32441, Alexa Fluor® 647 -
ab196144
, Alexa Fluor® 488 -
ab196368
, Carrier free -
ab220807
, PE -
ab303068
, APC -
ab303069
, HRP -
ab303070
, Alkaline Phosphatase -
ab308936
, Alexa Fluor® 594 -
ab310692
, Alexa Fluor® 555 -
ab312222
, Alexa Fluor® 568 -
ab312711
, Alexa Fluor® 750 -
ab321603
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please
contact us
for more information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SIRT1 also known as Silent mating type information regulation 2 homolog 1 is a NAD-dependent deacetylase enzyme. SIRT1 weighs approximately 120 kDa and plays an important role in regulating transcription apoptosis and stress resistance. Researchers have found SIRT1 in various tissues with higher expression in the heart brain and skeletal muscle. It is a component of the larger family of sirtuins which are involved in metabolic regulation and aging.
Biological function summary
SIRT1 modulates several cellular processes such as gene silencing DNA repair and lifespan extension. SIRT1 participates in complexes with other proteins including histones and transcription factors to influence chromatin structure and gene expression. It acts through deacetylation of target proteins affecting their function and stability. The activity of SIRT1 is also linked to environmental and cellular conditions including caloric intake and oxidative stress.
Pathways
SIRT1 is integral in the regulation of metabolic and longevity pathways. It interacts with the FOXO family proteins and the tumor suppressor protein p53 aiding in response to cellular stress and metabolic demands. The role of SIRT1 in the insulin signaling pathway exemplifies its influence on glucose homeostasis and energy balance. These interactions highlight its importance in metabolic health and aging.
SIRT1 links to neurodegenerative diseases such as Alzheimer's disease and metabolic disorders like type 2 diabetes. In Alzheimer's disease SIRT1 interacts with the amyloid precursor protein suggesting a protective role against amyloid-beta accumulation. Additionally studies have shown connections between SIRT1 and insulin receptor substrates highlighting its role in managing insulin sensitivity and glucose metabolism in diabetes. Understanding SIRT1's functions offers potential therapeutic targets for these disorders.