Abcam, ab32528, Anti-Plectin antibody [E398P]

Size: 100µL / 1mL
Anti-Plectin antibody [E398P] (ab32528) is a rabbit monoclonal antibody detecting Plectin in Western Blot, IHC-P . Suitable for Human . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 30 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:E398P,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody recognises plectin, a 500-kDa intermediate filament binding protein.

Product details:
What is this antibody validated in?
Anti-Plectin antibody [E398P] (ab32528) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.
What is the molecular weight of Plectin?
Anti-Plectin [E398P] (ab32528) specifically detects a band for Plectin (UniProt: Q15149) at a molecular weight of 532kDa.
Trusted by the scientific community
Anti-Plectin [E398P] (ab32528) was first used in a scientific publication in 2006 and has been cited over 30 times in peer-reviewed journals.
Reviewed by scientists
Anti-Plectin [E398P] (ab32528) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
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Specificity confirmed
The specificity of Anti-Plectin antibody [E398P] (ab32528) has been confirmed by Western blot testing in PLEC Knockout U2OS cell line.
Other related products
We have a range of other formats of antibody clone [E398P] also available for your convenience: ab32528, Carrier free -
ab176475
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Plectin also known as PLEC is a large protein with a molecular mass of approximately 500 kDa. It serves as a cross-linking element that plays an important role in maintaining the structural integrity of various cells. Plectin is widely expressed in tissues such as muscle skin and nervous tissue. Its mechanical function involves linking intermediate filaments to membrane complexes and other cytoskeletal structures which supports cellular architecture and stability.
Biological function summary
Plectin contributes significantly to the structural organization of cells and tissues. It is a part of the cytoskeletal complex and assists in cellular integrity by interacting with actin microfilaments microtubules and intermediate filaments. Plectin provides elasticity to cells allowing them to withstand mechanical stress. It ensures proper cellular functions and structural dynamics making it indispensable for maintaining tissue homeostasis.
Pathways
Plectin integrates into cellular signaling networks that control cytoskeletal organization and cell-matrix interactions. It plays a role in the integrin-mediated signaling pathway facilitating communication between the extracellular matrix and the cytoskeleton. Plectin interacts with proteins such as dystrophin which is involved in muscular pathways contributing to cell adhesion and motility.
Mutations or defects in the plectin gene can lead to conditions like epidermolysis bullosa simplex and muscular dystrophies. These disorders illustrate the important role that plectin plays in maintaining structural cell stability. In muscular dystrophies the interaction between plectin and dystrophin gets disrupted affecting muscle fiber integrity and leading to muscle weakness or degeneration. Such associations highlight plectin's significance in pathophysiological contexts.