Abcam, ab32572, Anti-beta Catenin antibody [E247] - ChIP Grade

Size: 20µL / 100µL / 1mL
Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) is a rabbit monoclonal antibody detecting beta Catenin in Western Blot, IP, IHC-P, ICC/IF, ChIP, mIHC . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency - Over 1070 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:E247,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, ChIP, ChIC/CUT&RUN-seq, IP, WB, ICC/IF, mIHCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is not suitable for ICC testing in mouse and rat species.Our inhouse testing indicated that this antibody does not work in Raw264.7 cell line in western blot. We have an alternative antibody ab68183 detecting weak band in lower expressor Raw264.7.

Product details:
Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIP, ICC/IF, IHC-P, IP and WB.
Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) was first used in a scientific publication in 1999 and has been cited over 1076 times in peer reviewed journals. It's performance in Western Blot in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) has been confirmed by Western Blot testing in beta Catenin knockout HepG2 cells (
ab277911
Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) has 75 independent reviews from customers.
Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) specifically detects beta Catenin (UniProt ID: P35222; Molecular weight: 86kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone E247 -
ab196204
Antibody clone E247 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
647, HRP, Alexa Fluor
594, Alexa Fluor
ab194118
ab194119
ab194120
ab201771
ab202496
Top cited antibody on the market with >1370 citations. E247 is top rated with >75 reviews. Beta-catenin plays a crucial role in cell adhesion and gene transcription and its dysregulation is linked to various cancers and diseases including, myocardial infarction, arrhythmias, cardiomyopathie and Alzheimers disease
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta Catenin also known by names such as CTNNB1 or beta-chip is an important protein involved in cell signaling and adhesion. This protein has a molecular weight of around 88 kDa. Beta Catenin is expressed in many cell types and tissues indicating its widespread role in various biological processes. It functions mechanically by mediating the linkage between cadherins and the actin cytoskeleton facilitating cell-cell adhesion. Beta Catenin is also a central part of transcription regulation processes in the nucleus.
Biological function summary
This protein plays roles in both cell adhesion and the regulation of gene expression. Beta Catenin is a critical component of the Wnt signaling pathway where it can form complexes with other proteins to influence gene transcription. In the absence of Wnt signaling beta Catenin levels are low due to its degradation. However when the pathway is active it accumulates in the cytoplasm and eventually translocates to the nucleus where it interacts with TCF/LEF transcription factors to regulate the expression of target genes.
Pathways
Beta Catenin plays a central role in the Wnt signaling pathway and influences cell fate decisions and cellular proliferation. It acts in concert with proteins such as Dishevelled (DVL) and Axin to coordinate these important biological processes. In the absence of Wnt signaling proteins such as APC and GSK-3β are responsible for beta Catenin degradation keeping its cellular levels in check. Beta Catenin's interaction with transcription factors in the nucleus makes it pivotal in the regulation of cell and tissue homeostasis.
Beta Catenin has associations with colorectal cancer and hepatocellular carcinoma. Its dysregulation can lead to unchecked cell proliferation and tumorigenesis. Often mutations in the beta Catenin gene (CTNNB1) or components of the Wnt pathway like APC are implicated in the development of these cancers. Its interplay with E-cadherin is important for maintaining tissue architecture and disruptions can lead to invasive cancer phenotypes. Understanding beta Catenin's role provides insights into therapeutic strategies for these cancers.