Abcam, ab32577, Anti-Caveolin-1 antibody [E249] - Caveolae Marker

Size: 20µL / 100µL / 1mL
Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577) is a rabbit monoclonal antibody detecting Caveolin-1 in Western Blot, Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:E249,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, IHC-P, Flow Cyt, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody should recognize both alpha and beta form of Caveolin-1.

Product details:
What is this antibody validated in?
Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of Caveolin-1?
Anti-Caveolin-1 [E249] - Caveolae Marker (ab32577) specifically detects a band for Caveolin-1 (UniProt: Q03135) at a molecular weight of 20kDa.
Trusted by the scientific community
Anti-Caveolin-1 [E249] - Caveolae Marker (ab32577) was first used in a scientific publication in 2006 and has been cited over 20 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl.
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Specificity confirmed
The specificity of Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577) has been confirmed by Western blot testing in CAV1 Knockout HeLa cells.
Other related products
We have a range of other formats of antibody clone [E249] also available for your convenience: ab32577, Alexa Fluor
ab185043
, Alexa Fluor
ab193892
, HRP -
ab193893
, Carrier free -
ab230262
, APC -
ab310857
, PE -
ab310925
, Alexa Fluor
ab311728
, Alexa Fluor
ab313005
, Alexa Fluor
ab313209
, Alexa Fluor
ab321611
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Caveolin-1 also known as Cav-1 is an integral membrane protein with a molecular weight of approximately 22 kDa. It functions mechanically as a scaffolding protein in caveolae which are tiny invaginations in the plasma membrane of many cell types. These structures are particularly abundant in adipocytes endothelial cells and muscle cells. Caveolin-1 anchors itself to the cell membrane and plays a role in organizing and concentrating certain signaling molecules.
Biological function summary
Caveolae provide a platform for various signaling pathways and involve caveolin-1 as a major component. Caveolin-1 interacts with multiple signaling molecules such as G-protein coupled receptors and Src family kinases to modulate signal transduction. This protein forms part of a larger caveolar complex contributing to cellular processes including endocytosis and lipid regulation. Its presence as a marker in caveolae highlights its significance in cellular functions.
Pathways
Caveolin-1 influences the insulin signaling and nitric oxide (NO) signaling pathways. In the insulin signaling pathway caveolin-1 interacts with insulin receptors to modulate glucose uptake. It also associates with eNOS (endothelial nitric oxide synthase) in the NO signaling pathway impacting vascular function. These relationships highlight its role in cellular communication and regulatory mechanisms within the human body.
Caveolin-1 is involved in cancer and cardiovascular diseases. In cancer altered expression of caveolin-1 contributes to tumor progression while in cardiovascular diseases it affects endothelial function through its interaction with eNOS. The connection of caveolin-1 to proteins like insulin receptors and eNOS reveals its critical influence in these conditions providing potential targets for therapeutic intervention.