Abcam, ab3373, Anti-MRP2 antibody [M2 III-6]

Size: 250µL
Anti-MRP2 antibody [M2 III-6] (ab3373) is a mouse monoclonal antibody detecting MRP2 in Western Blot . Suitable for Human . - KO validated for confirmed specificity - Over 80 publications - Trusted since 2003
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:M2 III-6,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ABCC2 aa 1300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q92887,
Epitope:This antibody reacts with an internal epitope of MRP 2.,
Specificity:This antibody detects MRP 2. It does not cross-react with human MDR 1, MRP 1, MRP 3 or MRP 5 gene products.

Product details:
What is this antibody validated in?
Anti-MRP2 antibody [M2 III-6] (ab3373) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.
What is the molecular weight of MRP2?
Anti-MRP2 [M2 III-6] (ab3373) specifically detects a band for MRP2 (UniProt: Q92887) at a molecular weight of 174kDa.
Trusted by the scientific community
Anti-MRP2 [M2 III-6] (ab3373) was first used in a scientific publication in 2003 and has been cited over 80 times in peer-reviewed journals.
Reviewed by scientists
Anti-MRP2 [M2 III-6] (ab3373) has over 5 independent reviews from customers.
Specificity confirmed
The specificity of Anti-MRP2 antibody [M2 III-6] (ab3373) has been confirmed by Western blot testing in ABCC2 Knockout A549 cells.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MRP2 also known as ABCC2 or MRP-2 transporter is an ATP-binding cassette transporter. This protein with a molecular mass of about 190 kDa is widely expressed in tissues such as the liver kidney and intestine. MRP2 plays a role in drug and metabolite transport moving various organic anions across cellular membranes. It locates to the canalicular membrane of hepatocytes where it functions in secreting conjugated bilirubin and drugs into bile.
Biological function summary
MRP2 contributes to cellular detoxification processes. It does not form part of a larger protein complex but works as an independent entity. MRP2 protein actively transports conjugated substances out of cells protecting tissues from potential damage from toxins drugs and other organic anions. This transporter mediates the excretion of glucuronide sulfate and glutathione conjugates playing an important role in phase III of drug metabolism.
Pathways
MRP2 transporter is integral to xenobiotic metabolism. It interconnects with the conjugation pathways that prepare compounds for excretion. The pathway functions with cytochrome P450 enzymes and other related transport proteins like MDR1 facilitating the secretion of detoxified metabolites. MRP2 activity influences pharmacokinetics and pharmacodynamics by modulating drug disposition and excretion.
MRP2 is associated with Dubin-Johnson syndrome and cholestasis. Dubin-Johnson syndrome arises from mutations in the ABCC2 gene leading to defective bilirubin excretion and jaundice. Cholestasis involves impaired bile excretion where MRP2 and possibly related proteins like BSEP play roles in bile salt export. Understanding MRP2 functioning aids in assessing risk and treatment strategies for these conditions.