Abcam, ab33752, Anti-MHC class I RT Ia antibody [OX-18]

Size: 100µg
Mouse Monoclonal MHC class I RT Ia antibody. Suitable for IP, Flow Cyt, ELISA, IHC-Fr and reacts with Rat samples. Cited in 4 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:OX-18,
Isotype:IgG1,
Carrier free:No,
Reacts with:Rat,
Applications:ELISA, IHC-Fr, Flow Cyt, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:Ab33752 recognises a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognised.

Product details:
This clone (OX-18) has been used in immunoaffinity purification of rat MHC class I molecules.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-This antibody was purified tissue culture supernatant., Storage buffer-pH: 7.4Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MHC class I RT Ia also known as RT1-A1 mechanically presents peptide antigens to CD8+ T cells. This protein has a mass of approximately 45 kDa. Expression of this protein occurs widely across various cell types including most nucleated cells. It plays an important role in the immune surveillance mechanism by displaying antigenic peptides on the cell surface for recognition by T-cell receptors.
Biological function summary
MHC class I RT Ia interacts with peptides within the endoplasmic reticulum and forms a complex with beta-2 microglobulin. This interaction is essential for the stability of the peptide-MHC complex on the cell surface. The formation of these complexes allows for immune recognition and establishment of immune responses. MHC class I RT Ia therefore plays an important role in initiating immune reactions against intracellular pathogens such as viruses.
Pathways
MHC class I RT Ia participates in the antigen processing and presentation pathway a fundamental aspect of adaptive immunity. It interacts with the transporter associated with antigen processing (TAP) proteins which transport peptides to the endoplasmic reticulum. Apart from that it also engages in the pathway involving cytotoxic T lymphocyte activation where it directly influences T-cell receptor signaling.
MHC class I RT Ia connects to autoimmune diseases like type 1 diabetes and ankylosing spondylitis. Variations in the genes encoding for MHC class I molecules may predispose individuals to these conditions. Additionally it’s associated with the HIV-1 virus where the virus may escape immune detection by downregulating MHC class I molecules presenting challenges in immune response which affect viral loads and disease progression.