Abcam, ab34710, Anti-Collagen I + Collagen III antibody

Size: 100µg
Anti-Collagen I + Collagen III antibody (ab34710) is a rabbit polyclonal antibody detecting Collagen I in Western Blot, IHC-P, ICC/IF . Suitable for Human . - Over 1850 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:This product was produced with the following immunogens:Native Full Length Protein corresponding to Human COL1A2. Database linkP08123Native Full Length Protein corresponding to Cow Collagen alpha-2(I) chain. Database linkP02465Native Full Length Protein corresponding to Cow COL1A1. Database linkP02453,
Specificity:This product is not recommended for use under denaturing conditions in WB. We would suggest testing it under native conditions. Denaturing and reducing conditions will greatly diminish reactivity and selectivity of this antibody. Abcam does not test ab34710 with endogenous samples in WB. We do recommend to look at the guidelines for blotting large proteins.Customers have been successful using ab34710 in this application, please see reference Tillgren V et al. J Biol Chem 290:918-25; 2015.Positive Control: Human stomach, skin and adrenal gland tissue lysates.

Product details:
Anti-Collagen I + Collagen III antibody (ab34710) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P and WB.
Anti-Collagen I + Collagen III antibody (ab34710) was first used in a scientific publication in 1977 and has been cited over 1859 times in peer reviewed journals. It's performance in Western blot and IHC in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Collagen I + Collagen III antibody (ab34710) has high sensitivity and specificity.
Anti-Collagen I + Collagen III antibody (ab34710) has 88 independent reviews from customers.
Anti-Collagen I + Collagen III antibody (ab34710) specifically detects Collagen I + Collagen III (UniProt ID: P02452; P08123; Molecular weight: 95kDa; 94kDa) and is sold in 100 µg selling sizes.
Top cited antibody to Collagen I and Collagen II on the market with >2200 citations. This reagent has >58 5 star reviews. Collagen I and Collagen III are essential for studying tissue structure, repair and fibrosis, aiding in research on skin, muscle and cardiovascular health.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-COLLAGEN I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens., Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Collagen I also known as collagen type 1 is a protein that provides mechanical support to tissues. It forms large fibrils and has a molecular mass of about 300 kilodaltons. Collagen I is expressed in connective tissues including skin bones tendons and ligaments. It serves as a structural scaffold that imparts tensile strength and stability. This protein is essential for maintaining the integrity of extracellular matrices in these tissues.
Biological function summary
Collagen I plays a central role in tissue architecture and wound healing. It is part of a larger collagen family comprising fibers that form a network within the extracellular space. In addition to its structural functions collagen I interacts with cells influencing processes like cell adhesion and migration. It undergoes remodeling through enzymes such as collagenases during tissue repair. Collagen I engages with various matrix proteins reinforcing its function in maintaining tissue homeostasis.
Pathways
Collagen I is involved in signaling pathways related to extracellular matrix organization and remodeling. Its interactions within the transforming growth factor-beta (TGF-β) pathway highlight its role in regulating tissue repair and fibrosis. Within this pathway collagen I associates with other proteins like fibronectin. In this context matrix metalloproteinases also relate to collagen I as they modify its structure and influence matrix dynamics.
Collagen I changes associate strongly with fibrosis and osteogenesis imperfecta. In fibrosis excessive collagen I accumulation leads to scarring and tissue function impairment. Proteins like TGF-β influence abnormal collagen I deposition in this context. In osteogenesis imperfecta mutations in collagen I genes disrupt its function affecting bone development and strength. Osteocalcin another bone-associated protein interacts with collagen I contributing to skeletal matrix formation.