Abcam, ab3529, Anti-Calcium Pump PMCA2 ATPase antibody

Size: 100µg
Rabbit Polyclonal Calcium Pump PMCA2 ATPase antibody. Suitable for ICC, IP, ELISA, WB, IHC-FoFr, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Pig, Chicken, Primates, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human ATP2B2 aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Primates, Chicken, Mouse, Pig, Rat,
Applications:ICC, IP, WB, ELISA, IHC-P, ICC/IF, IHC-Fr, IHC-FoFrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ATP2B2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.Q01814,
Specificity:Detects PMCA 2a and 2b ATPase.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Calcium Pump PMCA2 ATPase also known as PMCA2 or Ca-ATPase pump plays an important role in the active transport of calcium ions from the cytoplasm across the plasma membrane. It functions by hydrolyzing ATP to provide the energy needed for this process. PMCA2 belongs to the family of P-type ATPases and has a molecular mass of approximately 135 kDa. This pump is prominently expressed in the brain and inner ear particularly in the cerebellum and cochlea highlighting its importance in neuronal and auditory functions.
Biological function summary
PMCA2 regulates intracellular calcium concentration which is critical for cellular signaling and homeostasis. It does not form part of a larger complex but works independently to balance calcium levels. In neuronal cells efficient calcium clearance is necessary for synaptic activity and PMCA2 helps in maintaining the rapid calcium ion flux during neuronal firing. The presence of PMCA2 in auditory hair cells signifies its role in cochlear function and hearing sensitivity by aiding the processing of sound signals through calcium modulation.
Pathways
PMCA2 integrates into calcium signaling pathways and interacts with calmodulin which enhances its activity. Its function in the calmodulin-calcium signaling pathway is essential for processes such as neurotransmitter release and muscle contraction. In association with proteins like calmodulin PMCA2 ensures precise calcium dynamics safeguarding against cytotoxicity triggered by excessive calcium accumulation. The coordination with calmodulin formulates a pivotal connection within various signaling cascades.
PMCA2 mutations or dysfunctions have associations with neurological disorders and hearing impairments. Research links PMCA2 dysregulation to conditions such as spinocerebellar ataxia and hereditary hearing loss. In these scenarios PMCA2 interacts with molecular components that when impaired disrupt the normal calcium homeostasis. Such interruptions can lead to neurodegeneration or compromised auditory function underlining the significance of PMCA2 in maintaining health and preventing disease.