Abcam, ab3580, Anti-Glucocorticoid Receptor alpha antibody

Size: 50µg
Rabbit Polyclonal Glucocorticoid Receptor antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 34 publications. Immunogen corresponding to Synthetic Peptide within Human NR3C1 aa 750 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NR3C1 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P04150

Product details:
GR alpha proteins has many isoforms e.g. GR alpha-A, Alpha-2, GR-A alpha, Alpha-B. Please check Uniprot database or PMID 15866175 for more information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Glucocorticoid Receptor alpha also known as GR-alpha is a fundamental component in the family of nuclear receptors. It weighs approximately 97 kDa. This receptor is widely expressed in various tissues including the liver immune cells and the brain. The receptor functions through ligand-induced activation where it binds glucocorticoids. Upon binding GR-alpha undergoes a conformational change enabling it to translocate into the nucleus where it interacts with specific DNA sequences.
Biological function summary
The Glucocorticoid Receptor alpha influences numerous cellular processes like metabolism immune response and cell proliferation. It often functions as a part of larger protein complexes including transcriptional machinery. GR-alpha regulates gene expression by binding to glucocorticoid response elements (GRE) on DNA impacting the synthesis of specific proteins. This receptor modulates both positive and negative transcriptional control affecting various genes involved in biological responses.
Pathways
GR-alpha plays an important role in the Hypothalamic-Pituitary-Adrenal (HPA) axis and the inflammatory response pathway. In the HPA axis GR-alpha regulates cortisol synthesis and secretion impacting stress response and maintaining homeostasis. Within the inflammatory response GR-alpha interacts with NF-kB a protein complex that controls transcription of DNA to inhibit pro-inflammatory gene expression. These interactions exemplify GR-alpha’s regulatory influence on inflammation and stress-related responses.
GR-alpha is implicated in various conditions such as Cushing's syndrome and rheumatoid arthritis. In Cushing's syndrome impaired function or expression of GR-alpha leads to uncontrolled cortisol levels resulting in multiple metabolic and physiological disturbances. In rheumatoid arthritis GR-alpha mediates anti-inflammatory effects which are important for disease management. Connections between GR-alpha and pro-inflammatory cytokines emphasize its role in modulating inflammatory responses in these diseases.