Abcam, ab37149, Anti-ATF6 antibody

Size: 100µg
Anti-ATF6 antibody (ab37149) is a rabbit polyclonal antibody detecting ATF6 in Western Blot, IHC-P . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Over 100 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Based on 100% sequence alignment, this antibody detects ATF6 isoform A (alpha). The antibody is predicted to recognize the 50kDa fragment as it recognizes a peptide sequence from near the amino terminus. We have not tested stressed cells to see if the 50kDa cleavage product can be detected.

Product details:
What is this antibody validated in?
Anti-ATF6 antibody (ab37149) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.
What is the molecular weight of ATF6?
Anti-ATF6 (ab37149) specifically detects a band for ATF6 (UniProt: P18850) at a molecular weight of 75kDa.
Trusted by the scientific community
Anti-ATF6 (ab37149) was first used in a scientific publication in 2006 and has been cited over 100 times in peer-reviewed journals.
Reviewed by scientists
Anti-ATF6 (ab37149) has over 5 independent reviews from customers.
Specificity confirmed
The specificity of Anti-ATF6 antibody (ab37149) has been confirmed by Western blot testing in ATF6 Knockout HeLa cells.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Immunoaffinity purified, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Activating Transcription Factor 6 (ATF6) also known as ATF 6 is a significant protein in the endoplasmic reticulum (ER) stress response. The ATF6 protein has a molecular weight of approximately 84 kDa. It expresses predominantly in the endoplasmic reticulum membrane and plays a critical role in the unfolded protein response (UPR). During ER stress ATF6 relocates to the Golgi apparatus where it undergoes cleavage to become an active transcription factor. This process helps the cell to manage the accumulation of unfolded proteins.
Biological function summary
ATF6 operates as part of the transcription regulation mechanisms responding to ER stress. The ATF6 protein is essential in managing the expression of chaperone genes and ER-associated degradation (ERAD) components thereby maintaining protein homeostasis. ATF6 itself does not operate within a traditional complex but its activation involves proteolytic cleavage which subsequently releases the active form to the nucleus where it influences gene expression to alleviate stress conditions.
Pathways
ATF6 is prominently involved in the unfolded protein response pathway which manages cell survival and stress adaptation. This pathway closely interacts with other proteins like IRE1 and PERK forming a network that modulates the transcription of UPR target genes. Additionally ATF6 contributes to checkpoint control pathways that stabilize cellular environment by regulating genes related to chaperone and protein folding.
Malfunction or dysregulation of ATF6 links to conditions like diabetes and neurodegenerative diseases. In diabetes improper ATF6 function affects insulin signaling and glucose homeostasis often alongside proteins such as XBP1. In neurodegenerative diseases an inappropriate response to ER stress influences the progression of disorders like Alzheimer's disease where protein misfolding and aggregation play pivotal roles. Understanding how ATF6 interplays with these diseases could lead to new therapeutic avenues.