Abcam, ab3891, Anti-ARIH1 antibody

Size: 100µg
Goat Polyclonal ARIH1 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human ARIH1 aa 500 to C-terminus.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ARIH1 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9Y4X5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: 0.5% BSA, 0.5% Tris, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ARIH1 also known as Human Ubiquitin-Protein Ligase ARIH1 is a 55-kDa protein involved in the ubiquitination process. Ubiquitination is a process that tags proteins for degradation. ARIH1 functions as an E3 ubiquitin-protein ligase facilitating the transfer of ubiquitin to specific substrate proteins. It acts with ubiquitin-conjugating enzymes mediating the attachment of ubiquitin molecules to protein targets. ARIH1 is mainly expressed in the cytoplasm and nucleus of cells with higher expression detected in the brain and testes.
Biological function summary
ARIH1 plays a role in the regulation of several cellular processes such as protein degradation signal transduction and DNA repair. It participates in the formation of the ARIH1-E2 ubiquitin-protein ligase complex which modulates protein stability by marking them for proteasomal degradation. This complex ensures the controlled turnover of key regulatory proteins to maintain cellular homeostasis. ARIH1 also influences the function of other important molecules through its ubiquitination activity impacting cellular responses to stress.
Pathways
ARIH1 gets involved in the ubiquitin-proteasome system which orchestrates the degradation of unneeded or damaged proteins. ARIH1 operates within this pathway interacting with proteins like the E2 conjugating enzyme UBE2L3. Moreover it participates in the NEDDylation pathway which adds another layer of regulation to protein stability and function. Within these pathways ARIH1 ensures effective control over multiple cellular mechanisms thereby supporting cellular function.
ARIH1 associates with conditions like cancer and neurodegenerative diseases. In cancer altered ubiquitination processes involving ARIH1 may disrupt normal cellular regulation contributing to tumorigenesis. Additionally ARIH1's dysregulation impacts neurodegenerative diseases potentially linked to improper protein degradation in neurons. ARIH1's interactions with proteins such as p53 and PARK2 further connect it to these disorders highlighting its role in the pathological processes.