Abcam, ab39012, Anti-MMP13 antibody

Size: 100µg
Anti-MMP13 antibody (ab39012) is a rabbit polyclonal antibody detecting MMP13 in Western Blot, Flow Cytometry, IHC-P, IHC-Fr, ICC/IF, ELISA . Suitable for Dog, Human, Mouse, Rat . - Over 510 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Dog, Rat,
Applications:ICC/IF, WB, IHC-P, IHC-Fr, Flow Cyt, sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MMP13. The exact immunogen used to generate this antibody is proprietary information.P45452,
Specificity:ab39012 recognizes the latent proenzyme, at 60 Kd, as well as the active form at 48 Kd, and intermediate activation forms. It does not cross react with the other MMP family members. ab39012 recognizes the Hinge region of MMP13.

Product details:
Product Specifications
Anti-MMP13 antibody (ab39012) is a rabbit polyclonal antibody and is validated for use in Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB, sELISA in dog, human, mouse, rat samples.
Anti-MMP13 antibody (ab39012) specifically detects MMP13 (UniProt ID: P45452; Molecular weight: 42kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-MMP13 antibody (ab39012) has high sensitivity and specificity.
Anti-MMP13 antibody (ab39012) has been cited over 517 times in peer reviewed journals and is trusted by the scientific community.
Anti-MMP13 antibody (ab39012) has 18 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-The antibody has been peptide-affinity purified., Storage buffer-Preservative: 0.05% Sodium azideConstituents: 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Matrix metallopeptidase 13 (MMP-13) also called collagenase 3 is an enzyme with an approximate molecular mass of 54 kDa. It plays a significant role in the breakdown of extracellular matrix components particularly collagen. MMP-13 is expressed in several tissues including cartilage skin and bone. Its expression sees an increase during tissue remodeling and in pathological conditions. MMP-13 also participates in processes like wound healing and embryonic development making it a focal point for understanding tissue dynamics.
Biological function summary
Matrix metallopeptidase 13 contributes extensively to the degradation of collagen type II a major component of cartilage. As a zinc-dependent endopeptidase MMP-13 is part of the MMP family which facilitates the remodeling of the extracellular matrix. MMP-13 is involved in the proteolytic cascade working in conjunction with other MMPs and elastase to mediate tissue repair and turnover. It does not form complexes but acts in concert with other enzymes to execute its physiological functions effectively.
Pathways
Matrix metallopeptidase 13 significance is most noted in the cartilage degradation pathway. It interacts with other MMPs such as MMP-1 and MMP-9 to efficiently break down extracellular matrix components. These interactions highlight its role in the regulation of matrix metalloproteinase activity within the connective tissue degradation pathway. These pathways are key during normal connective tissue remodeling and in pathological processes illustrating the essential roles of MMPs in maintaining tissue homeostasis.
Matrix metallopeptidase 13 has strong associations with osteoarthritis and rheumatoid arthritis. It contributes to cartilage destruction intensifying the progression of these disorders due to its potent collagenolytic activity. Other proteins like MMP-9 and MMP-14 are also involved in these processes potentially amplifying tissue damage in affected joints. Understanding how MMP-13 and related proteins drive these diseases offers potential therapeutic targets for slowing disease progression and enhancing joint health.