Abcam, ab4055, Anti-CD8 alpha antibody

Size: 500µL / 1mL
Anti-CD8 alpha antibody (ab4055) is a rabbit polyclonal antibody detecting CD8 alpha in IHC-P . Suitable for Human . - Over 180 publications - Trusted since 2003
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab178089.

Product details:
Product Specifications
Anti-CD8 alpha antibody (ab4055) is a rabbit polyclonal antibody and is validated for use in IHC-P in human samples.
Anti-CD8 alpha antibody (ab4055) specifically detects CD8 alpha (UniProt ID: P01732; Molecular weight: 24kDa) and is sold in 500 µL and 1 mL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-CD8 alpha antibody (ab4055) has high sensitivity and specificity.
Anti-CD8 alpha antibody (ab4055) has been cited over 184 times in peer reviewed journals and is trusted by the scientific community.
Anti-CD8 alpha antibody (ab4055) has 14 independent reviews from customers.
Target Information
CD8 alpha is a transmembrane glycoprotein that serves as a co-receptor for the T-cell receptor (TCR), primarily found on cytotoxic T cells. It plays a crucial role in T cell signaling and cytotoxic activity by binding to MHC class I molecules, aiding in the immune response against infected or malignant cells. CD8 alpha is associated with several diseases, particularly those involving immune system dysfunction.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.1% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.
Biological function summary
The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.
Pathways
CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.
CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.