Abcam, ab43019, Anti-Mycobacterium tuberculosis Ag85B antibody

Size: 100µL
Anti-Mycobacterium tuberculosis Ag85B antibody (ab43019) is a rabbit polyclonal antibody detecting Mycobacterium tuberculosis Ag85B in Western Blot, IHC-P, ELISA . - Over 10 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mycobacterium tuberculosis,
Applications:WB, IHC-P, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody is specific for the Ag85B protein in sera, plasma or cell culture supernatant.

Product details:
What is this antibody validated in?
Anti-Mycobacterium tuberculosis Ag85B antibody (ab43019) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), ELISA.
What is the molecular weight of Mycobacterium tuberculosis Ag85B?
Anti-Mycobacterium tuberculosis Ag85B (ab43019) specifically detects a band for Mycobacterium tuberculosis Ag85B (UniProt: P9WQP1) at a molecular weight of 34kDa.
Trusted by the scientific community
Anti-Mycobacterium tuberculosis Ag85B (ab43019) was first used in a scientific publication in 2006 and has been cited over 10 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: 1.2114% Sodium citrate, 0.6057% Tris, 0.05% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Mycobacterium tuberculosis Ag85B protein also known as Antigen 85B or FBPA is a component of the Ag85 complex. This protein has a molecular mass of approximately 31 kDa. The Ag85B protein plays an important mechanical role by catalyzing the formation of the cord factor a major glycolipid in the cell wall of Mycobacterium tuberculosis. It is expressed in the mycobacterium's cell wall contributing to the virulence of this pathogen by promoting the invasion and survival within the host.
Biological function summary
The Ag85B protein serves in the assembly and maintenance of the mycobacterial cell wall by synthesizing mycolate. It is a part of the Ag85 complex which includes Ag85A Ag85B and Ag85C ensuring accurate synthesis and export of essential components of the cell wall. Through its actions Ag85B is involved in interacting with the host immune system and is a target for vaccine development due to its immunogenic properties. Its presence is detected during histological examinations of infected tissues where it shows the interaction with host cells.
Pathways
The Ag85B protein functions within the mycolic acid biosynthesis pathway essential for maintaining the structure of the mycobacterial cell envelope. This protein interacts with Ag85A and Ag85C to orchestrate complex lipid synthesis required for Mycobacterium tuberculosis' survival. Ag85B's role in lipid metabolism makes it important in the pathogen's adaptation to the host environment by altering the cell wall to evade immune detection.
Ag85B is highly relevant to respiratory diseases like tuberculosis. This protein plays a role in the pathogen's ability to resist destruction by the host's immune system contributing to the chronic nature of tuberculosis. Additionally Ag85B has been investigated in the context of its potential utility in vaccines and diagnostic tests for Mycobacterium tuberculosis infections. Its interactions with other proteins in the Ag85 complex make it a focus of research for developing therapeutics to treat and manage tuberculosis.