Abcam, ab45892, Human FAS Ligand ELISA Kit (CD95L)

Size: 1 x 96Tests / 2 x 96Tests
Human FAS Ligand ELISA Kit (CD95L) is a Sandwich (quantitative) ELISA for the measurement of Human FAS Ligand (CD95L) in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 12 pg/mL,
Range:62.5 - 2000 pg/mL,
Assay time:3h 45m,
Assay Platform:Microplate

Product details:
Abcam's Human FAS Ligand
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of FAS Ligand in Human serum, plasma, buffered solutions and cell culture media.
A monoclonal antibody specific for FAS Ligand has been coated onto the wells of the microtiter strips provided. Samples, including standards of known FAS Ligand concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for FAS Ligand are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of FAS Ligand present in the samples.
This kit will recognize both endogenous and recombinant Human FAS Ligand.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C