Abcam, ab46034, Human IL-10 ELISA Kit

Size: 1 x 96Tests / 2 x 96Tests
Human IL-10 ELISA Kit is a sandwich ELISA designed to quantify Human IL-10 with a sensitivity of 5 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 12.5 - 400 pg/mL - Cited in over 30 publications
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 5 pg/mL,
Range:12.5 - 400 pg/mL,
Assay time:2h 45m,
Assay Platform:Microplate

Product details:
Human IL-10 ELISA Kit ab46034 is a sandwich ELISA to measure Human IL-10 in serum, plasma, cell culture supernatant with a sensitivity of 5 pg/ml.
How the assay works
A monoclonal antibody specific for IL-10 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-10 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-10 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-10 present in the samples.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human IL-10 ELISA Kit ab46034 protocol summary
1. Add standard or sample to each well used
2. Add prepared Biotinylated labeled detector antibody. Incubate at room temperature
3. Aspirate and wash each well
4. Add prepared Streptavidin-HRP mix to each well. Incubate at room temperature
5. Aspirate and wash each well.
6. Add the TMB Solution to each well until color develops and then add the Stop Solution
7. Immediately begin recording the color development
Get results in 90 minutes with our SimpleStep ELISA
Human IL-10 ELISA Kit (
ab185986

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C