Abcam, ab46070, Rat TNF alpha ELISA Kit

Size: 1 x 96Tests / 2 x 96Tests
Rat TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat TNF alpha in Rat in Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Serum,
Reacts with:Rat,
Assay type:Sandwich (quantitative),
Sensitivity:< 15 pg/mL,
Range:31.25 - 1000 pg/mL,
Assay time:3h 45m,
Assay Platform:Microplate

Product details:
Abcam's TNF alpha Rat
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Rat TNF alpha in cell culture supernatants, serum and plasma samples.
A monoclonal antibody specific for TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.
This kit will recognize both endogenous and recombinant rat TNF alpha

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C