Abcam, ab46506, Human Syndecan-1 ELISA Kit (CD138)

Size: 1 x 96Tests / 2 x 96Tests
Human Syndecan-1 ELISA Kit (CD138) is a Sandwich ELISA used to quantify Human Syndecan-1 (CD138) with a sensitivity of 4.94 ng/ml - Colorimetric ELISA kit - 450 nm readout. Works on any standard plate reader - Wide dynamic range – 8 - 256 ng/ml - Cited in over 30 citations
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 4.94 ng/mL,
Range:8 - 256 ng/mL,
Assay time:1h 45m,
Assay Platform:Microplate

Product details:
Human Syndecan-1 ELISA (CD138) kit is designed for the quantitative measurement of Syndecan-1 in Human serum, plasma, buffered solutions and supernatants.
A monoclonal antibody specific for Syndecan-1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Syndecan-1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Syndecan-1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Syndecan-1 present in the samples.
This kit will recognize both endogenous and recombinant Human Syndecan-1.
Human Syndecan-1 ELISA Kit (CD138) ab46506 is a sandwich ELISA to measure Human Syndecan (CD138) in serum, plasma, buffered solutions and supernatants. with a sensitivity of 4.94 ng/mL.
How the assay works
A monoclonal antibody specific for Syndecan-1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Syndecan-1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Syndecan-1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Syndecan-1 present in the samples.
Assay specificity
This kit will recognize both endogenous and recombinant Human Syndecan-1.
Human Syndecan-1 ELISA Kit (CD138) ab46506 protocol summary
1. Add standard or sample to each well used
2. Add prepared Biotinylated labeled detector antibody. Incubate at room temperature
3. Aspirate and wash each well. Add prepared Streptavidin-HRP mix to each well. Incubate at room temperature
4. Aspirate and wash each well. Add the TMB Solution to each well until color develops and then add the Stop Solution. Immediately begin recording the color development
How other researchers are using Human Syndecan-1 ELISA Kit (CD138) ab46506
Human Syndecan-1 ELISA Kit (CD138) ab46506 has been used to measure endothelial glycocalyx degradation in children with sepsis
and in traumatic hemorrage
References
J Fernandez-Sarmiento et al. 2024, PMID:38245695
S. Abdullah et al. 2023, PMID: 37315138
Related and recommended products
Human Syndecan-1 ELISA Kit (CD138) ab46506 is commonly used to study inflammation, endothelial dysfunction and other cellular processes.
Other ELISA kits commonly use alongside Syndecan-1 include:
- Human VEGF ELISA Kit
ab222510
- Human IL-6Elisa Kit
ab178013
- Human MMP9 ELISA Kit
ab246539
- Human ICAM1 ELISA Kit (CD54)
ab174445

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Syndecan-1 also known as CD138 is a transmembrane heparan sulfate proteoglycan with a molecular weight of approximately 30 to 80 kDa. The size variation arises from differences in its glycosaminoglycan chains. This protein resides predominantly on the surface of epithelial cells and plasma cells. Researchers commonly use the CD138 marker to identify these cells in biological samples. It plays a significant role in cell-cell and cell-matrix interactions acting as a co-receptor for a variety of growth factors cytokines and extracellular matrix proteins.
Biological function summary
Syndecan-1 participates in tissue repair inflammation and cell signaling ensuring epithelial integrity and communication. Syndecan-1 influences cell behavior by interacting with other components of the extracellular matrix and different membrane receptors. It is also involved in forming and regulating proteoglycan complexes at the cell surface. These complex interactions facilitate the modulation of cell proliferation migration and differentiation which are fundamental to maintaining tissue architecture and function.
Pathways
Syndecan-1 actively contributes to both the Wnt and FGF signaling pathways. Through its interaction with proteins such as miR-15 syndecan-1 modulates cellular responses essential for developmental processes and tissue homeostasis. The connection with these pathways allows syndecan-1 to integrate and relay extracellular signals impacting gene expression and cellular behavior. Its relationship with other proteins like A38 as a co-receptor emphasizes its role in finely tuning signaling cascades within cellular environments.
Syndecan-1 shows a connection to multiple myeloma and breast cancer. The overexpression of syndecan-1 in these conditions disrupts normal cellular functions and promotes tumorigenesis by aiding in the tumor cell's adhesion migration and invasion. Its interaction with proteins such as perCP further demonstrates its role in disease progression providing potential as a therapeutic target. These relationships make syndecan-1 an important focus for research in oncology and other fields of disease study.