Abcam, ab46545, Anti-4 Hydroxynonenal antibody

Size: 100µg
Anti-4 Hydroxynonenal antibody (ab46545) is a rabbit polyclonal antibody detecting 4 Hydroxynonenal in Western Blot . - Over 650 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:Specifically binds to HNE modified proteins.

Product details:
Anti-4 Hydroxynonenal antibody (ab46545) is a rabbit polyclonal antibody and is validated for use in WB.
Anti-4 Hydroxynonenal antibody (ab46545) was first used in a scientific publication in 2008 and has been cited over 658 times in peer reviewed journals. It's performance in Western blot is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-4 Hydroxynonenal antibody (ab46545) has high sensitivity and specificity.
Anti-4 Hydroxynonenal antibody (ab46545) has 25 independent reviews from customers.
Anti-4 Hydroxynonenal antibody (ab46545) specifically detects 4 Hydroxynonenal (UniProt ID: N/A; Molecular weight: 0.1kDa) and is sold in 100 ug selling sizes.
4-Hydroxynonenal is essential for detecting oxidative stress markers in cells, aiding research on diseases related to oxidative damage.

Properties and Storage Information:
Form-Liquid, Purification notes-This antibody was purified by an HNE modified Protein-Sepharose affinity column., Storage buffer-pH: 7.2 - 7.4Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
4-Hydroxynonenal (4-HNE) often referred to as HNE is a highly reactive aldehyde with a molar mass of approximately 156.19 g/mol. It plays a significant role in lipid peroxidation processes. 4-HNE forms during the oxidative degradation of polyunsaturated fatty acids and is present mostly in tissues with high metabolic activity. It is frequently expressed in the liver brain and heart where oxidative stress is elevated. Researchers commonly use techniques like 4-HNE staining and 4-HNE Western blot to study its presence and implications in various tissues and cells.
Biological function summary
4-HNE serves as a signaling molecule influencing many cellular pathways. It is not just a byproduct of oxidative stress but actively modifies proteins through covalent binding. These modifications often lead to changes in protein function and structure affecting cellular processes such as proliferation apoptosis and differentiation. 4-HNE also interacts with other reactive molecules and antioxidants forming a complex network of cellular responses. This aldehyde regulates gene expression influencing how cells respond to oxidative stress and inflammation.
Pathways
4-HNE interacts with pathways involved in oxidative stress and detoxification. In the oxidative stress pathway it reacts with glutathione to form glutathione conjugates which are part of the cell’s detoxifying responses. The aldehyde thereby affects the Keap1-Nrf2-ARE pathway which modulates antioxidant responses and cellular protection against oxidative damage. Proteins like glutathione peroxidase and glutathione-S-transferase play key roles in these pathways illustrating the interconnectedness of 4-HNE with cellular defense mechanisms.
4-HNE has associations with conditions such as Alzheimer's disease and cardiovascular diseases. In Alzheimer's the aldehyde contributes to neuroinflammation and protein modification impacting neuronal function and survival. It interacts with proteins like tau and amyloid-beta which are critical in the pathology of Alzheimer's. In cardiovascular diseases 4-HNE mediates cellular damage and inflammation linking it to the progression of atherosclerosis. This reactive aldehyde interacts with proteins such as apolipoprotein B highlighting its significant role in disease pathways linked to oxidative stress.