Abcam, ab4680, Anti-Neurofilament heavy polypeptide antibody

Size: 25µL
Anti-Neurofilament heavy polypeptide antibody (ab4680) is a chicken polyclonal antibody detecting Neurofilament heavy polypeptide in Western Blot, IHC-Fr . Suitable for Cow, Human, Mouse, Rat . - Over 130 publications - Trusted since 2003
Key facts
Host species:Chicken,
Clonality:Polyclonal,
Isotype:IgY,
Carrier free:No,
Reacts with:Mouse, Rat, Cow, Human,
Applications:WB, ICC, IHC-FrFlSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-Neurofilament heavy polypeptide antibody (ab4680) is a Chicken Polyclonal antibody and is validated for use in ICC, IHC-FrFl, WB.
Anti-Neurofilament heavy polypeptide antibody (ab4680) has been cited over 135 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality validation processes ensure Anti-Neurofilament heavy polypeptide antibody (ab4680) has high sensitivity and specificity.
Anti-Neurofilament heavy polypeptide antibody (ab4680) has 12 independent reviews from customers.
Anti-Neurofilament heavy polypeptide antibody (ab4680) specifically detects Neurofilament heavy polypeptide (UniProt ID: P12036; Molecular weight: 112kDa) and is sold in 25 uL selling sizes.
Neurofilament heavy polypeptide (NEFH, NFH) is a critical component of the neurofilament network, essential for maintaining the structural integrity and function of neurons. It plays a significant role in the radial growth of axons and the transmission of electrical impulses. Alterations in NEFH expression are associated with various neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Charcot-Marie-Tooth disease, and Alzheimer's disease (AD). Studying NEFH helps researchers understand the pathology of these conditions and the underlying neuronal dysfunction, highlighting its importance in the broader context of neurodegenerative disease research.

Properties and Storage Information:
Form-Liquid, Purity-IgY fraction, Purification notes-This is an IgY prep, similar to a mammalian ammonium sulphate IgG prep from serum. It is virtually pure IgY and we estimate that about 5% of material is specific to the immunogen., Storage buffer-Preservative: 0.032% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Neurofilament heavy polypeptide often referred to as NF-H or neurofilament heavy chain plays an important role in the structural integrity of neurons. This protein is a critical component of the cytoskeleton in neurons consisting of an approximate molecular mass of 200 kDa. It is predominantly expressed in the neuronal axons where it contributes to the assembly and stability of neurofilament structures. NF-H is essential for maintaining axonal diameter and for proper conduction of electrical impulses along nerve fibers.
Biological function summary
This polypeptide participates in the formation of neurofilament a complex structure that includes other neurofilament proteins like the neurofilament light (NF-L) and neurofilament medium (NF-M). These proteins form a stable scaffold within the axon aiding in neuronal growth and repair. The phosphorylation of NF-H modulates its interactions within the neurofilament network influencing axonal transport processes and dynamics which are vital for neuron function and survival.
Pathways
Neurofilament heavy polypeptide is integral to the axonal transport pathway important in conveying molecules between the neuronal cell body and the synaptic terminals. Through its interactions with microtubules and motor proteins NF-H facilitates the movement of organelles and neurotransmitters. It has significant associations with proteins involved in the ubiquitin-proteasome system reflecting its role in maintaining neuronal homeostasis and proteostasis essential in neuronal development and function.
Neurofilament heavy polypeptide serves as an important biomarker for neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. NF-H especially in phosphorylated form accumulates in conditions of axonal injury or degeneration. It often interacts with tau protein in Alzheimer's pathology forming neurofibrillary tangles. Elevated levels of NF-H in cerebrospinal fluid or blood can indicate neuronal damage offering insights into disease progression and potential targets for therapeutic intervention.