Abcam, ab47118, Anti-SPLASH antibody

Size: 100µg
Rabbit Polyclonal SPLASH antibody. Suitable for ELISA, WB, FuncS, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PLA2G2D.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WB, FuncS, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human PLA2G2D.Q9UNK4,
Specificity:This antibody is specific for SPLASH.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Immunoaffinity chromatography on a column with immobilized recombinant human SPLASH., Storage buffer-Constituents: PBS, 0.58% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SPLASH protein also known as Synapse Protein LL Alpha SH3 functions as an adaptor in synaptic signaling. A molecular weight of approximately 50 kDa characterizes SPLASH and it shows high expression in neural tissues notably within the brain's synaptic regions. Synapse formation and plasticity strongly depend on the presence and function of this protein. It interacts with multiple synaptic components to assist in the organization of signal transduction complexes.
Biological function summary
The SPLASH protein coordinates with other synaptic proteins to facilitate neurotransmitter release and receptor clustering at the synapse. As part of larger protein complexes SPLASH forms dynamic interactions important for maintaining synaptic strength and plasticity. These complexes modulate synaptic vesicle exocytosis and endocytosis processes which are essential for efficient neuronal communication.
Pathways
The SPLASH protein integrates within the synaptic signaling pathway and the calcium signaling pathway. It serves as a link between the two pathways coordinating signal transduction events that modulate synaptic plasticity. SPLASH maintains functional connection with proteins like SNARE and PSD-95 which play central roles in synaptic vesicle fusion and postsynaptic density organization respectively.
The SPLASH protein links to neurodevelopmental disorders and synaptic dysfunction. It is particularly associated with conditions such as autism spectrum disorder where synaptic signaling and plasticity are affected. Moreover in Alzheimer's disease altered SPLASH expression influences amyloid precursor protein processing through its interactions with the Tau protein impacting cognitive function and neuronal integrity.