Abcam, ab4775, Anti-EIF2B5 (phospho S539) antibody

Size: 50µL
Rabbit Polyclonal EIF2B5 phospho S539 antibody. Suitable for WB and reacts with Recombinant full length protein - Rat, Mouse, Rat samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Rat Eif2b5 phospho S539.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Rat Eif2b5 phospho S539. The exact immunogen used to generate this antibody is proprietary information.Q64350,
Specificity:This ab reacts with both human and rat (see, for example, PMID 21165642, 20484009 and 21832246) but it is not very clear if this recognition happens only when S535 (rat) and S544 (human) are phosphorylated or when S535 (rat)/S540 (human) are phosphorylated.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively pre-adsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated eukaryotic initiation factor 2B epsilon (eIF-2B epsilon), and (ii) a generic serine phosphorylated peptide to remove antibody that is reactive with phospho-serine, irrespective of the sequence. The final product is generated by affinity chromatography using an eIF-2B epsilon-derived peptide that is phosphorylated at serine 539., Storage buffer-pH: 7.3Preservative: 0.05% Sodium azideConstituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EIF2B5 also known as eukaryotic translation initiation factor 2B subunit epsilon plays an important role in the regulation of protein synthesis. It is a catalytic subunit of the larger eIF2B complex which is responsible for guanine nucleotide exchange on eIF2. This protein has a molecular mass of approximately 85 kDa. EIF2B5 is ubiquitously expressed indicating its importance in various tissues. Its role in translation initiation is fundamental in controlling cellular responses to stress and nutrient signaling.
Biological function summary
Protein synthesis control by eIF2B5 impacts cell growth and adaptation. As an important component of the eIF2B complex which consists of five different subunits EIF2B5 directly interacts with eIF2 to facilitate GDP-GTP exchange. This process is essential for recycling eIF2 for successive rounds of translation initiation. Proper function of the eIF2B complex ensures cellular capacity to manage stress conditions such as amino acid deprivation or viral infection by modulating protein synthesis rates to conserve resources and adapt.
Pathways
EIF2B5 plays a significant role in the integrated stress response (ISR) pathway. Through its involvement in ISR it links with proteins like eIF2 and integrates signaling from different stressors. This pathway helps in adjusting the translation of specific mRNAs by phosphorylating eIF2 making EIF2B5 essential for the regulation of global and gene-specific translation. Additionally dysfunctions in its regulation may impact the mTOR pathway an important regulator of cell growth in response to nutrient availability although EIF2B5 mainly functions in ISR.
Mutations in EIF2B5 are associated with leukoencephalopathy with vanishing white matter (VWM) a rare neurological disorder. This condition links EIF2B5 dysfunction to impaired oligodendrocyte maturation and myelination in the brain. Additionally altered functioning of EIF2B5 may relate to other stress-related disorders as its regulatory role in translation impacts cell survival under stress conditions. Connections between eIF2B5 and proteins like eIF2 underline its involvement in proper neuronal function and response to metabolic changes important to understanding disorders like VWM.