Abcam, ab49024, Recombinant Hepatitis C Virus NS3 protein

Size: 100µg
Recombinant Hepatitis C Virus NS3 protein is a Hepatitis C virus (isolate BK) Fragment protein, in the 1359 to 1456 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, ELISA, WB, Flow Cyt.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:GST tag N-Terminus,
Applications:ELISA, WB, SDS-PAGE, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P26663,
Animal free:No,
Carrier free:No,
Species:Hepatitis C virus (isolate BK),
Storage buffer:pH: 7.2 - 7.6Constituents: 50% Glycerol (glycerin, glycerine), 9% Urea, 0.395% Tris HCl, 0.2% Triton X-100

Product details:
Strongly reacts with human HCV positive serum.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Hepatitis C Virus NS3 also known as non-structural protein 3 is an important component of the Hepatitis C viral replication machinery. It functions as a serine protease and RNA helicase necessary for processing the HCV polyprotein and unwinding RNA respectively. The NS3 protein has a molecular mass of approximately 70 kDa and is expressed primarily in infected hepatocytes. It often comes up in research discussions during NS3 conferences and is a focus of many studies seeking to understand HCV replication mechanisms.
Biological function summary
NS3 plays a significant role in the life cycle of Hepatitis C Virus by facilitating viral replication and maturation. NS3 protease activity cleaves the non-structural protein precursors at multiple sites which is essential for the formation of the viral replication complex. As part of the HCV replication complex it associates with the NS4A cofactor that enhances its protease function. The protein is highly conserved and represents an attractive target for antiviral drug development due to its indispensable role in the viral life cycle.
Pathways
NS3 is integral to the viral replication and host immune evasion pathways. The protein interacts in the post-translational modification processing pathway that is critical for producing functional viral proteins. NS3 activity is closely related to other non-structural proteins such as NS5A and NS5B which are involved in RNA replication. These interactions form a network essential for the hepatitis C infection propagation influencing the virus's ability to hijack host cells' functions.
NS3 is directly associated with Hepatitis C a significant cause of chronic liver disease including cirrhosis and hepatocellular carcinoma. NS3's activity contributes to the persistence of the infection often evading host immune responses. The protein plays a role in the pathogenesis of Hepatitis C by modulating immune signaling pathways. NS3 also interacts with host proteins such as TLR3 involved in antiviral responses thereby impacting disease progression and leading to potential long-term liver damage. Researchers target NS3 in the development of direct-acting antivirals aiming to disrupt its enzymatic functions to curb Hepatitis C infection.