Abcam, ab49577, Biotin Anti-Urokinase antibody

Size: 150µg
Rabbit Polyclonal Urokinase antibody - conjugated to Biotin. Suitable for IP, ELISA, WB, RIA and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human Urokinase-type plasminogen activator.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Conjugation:Biotin,
Carrier free:No,
Reacts with:Human,
Applications:WB, IP, RIA, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Human Urokinase-type plasminogen activator.P00749

Product details:
ATF is the amino terminal fragment of urokinase (uPA) corresponding to amino acid residues 1-135. uPA-ATF contains the growth factor like domain, which binds to the urokinase receptor, and the kringle domain which is involved in the induction of cell chemotaxis by urokinase. ATF inhibits the binding of urokinase to its receptor uPAR and acts as a broad inhibitor of cell migration.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.25% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Urokinase also known as urokinase-type plasminogen activator (uPA) is a serine protease with a molecular mass of approximately 54 kDa. It is expressed in various tissues including the kidneys liver and blood vessels. Urokinase initially forms as pro-urokinase an inactive precursor that can convert into the active enzyme. Researchers often use assays like the direct assay to measure urokinase activity due to its role in breaking down fibrin and modulating cell adhesion and migration.
Biological function summary
Something particularly interesting about urokinase is its role in converting plasminogen to plasmin a critical enzyme in the process of fibrinolysis. Moreover it is part of a larger enzymatic complex involving the urokinase receptor (uPAR) which localizes plasminogen activation to cell surfaces. This complex mediates extracellular matrix degradation aiding in important cellular processes like tissue remodeling and wound healing.
Pathways
Urokinase plays a critical role in the fibrinolytic pathway which involves the breakdown of blood clots. It also interacts with the extracellular matrix pathway by activating plasminogen which can degrade matrix components. In these pathways urokinase closely interacts with proteins such as plasmin and its inhibitor plasminogen activator inhibitor-1 (PAI-1) which regulate the activity and localization of proteolytic events.
Urokinase is strongly linked to conditions such as deep vein thrombosis and cancer metastasis. Its overexpression or dysregulation often leads to excessive tissue degradation influencing metastasis progression. Urokinase is connected to the urokinase receptor (uPAR) in cancer facilitating cancer cell invasion and migration. Understanding its behavior in these contexts is critical for developing therapeutic strategies and targeting treatments against these diseases.