Abcam, ab5, Anti-CENPF antibody

Size: 50µL
Anti-CENPF antibody (ab5) is a rabbit polyclonal antibody detecting CENPF in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human, Mouse . - Over 100 publications - Trusted since 1998
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:WB, IP, ICC, ICC/IF, Flow Cyt, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Fusion protein corresponding to Centromere protein F, fused to Centromere protein F. The exact immunogen used to generate this antibody is proprietary information.P49454

Product details:
Product Specifications
Anti-CENPF antibody (ab5) is a Rabbit Polyclonal antibody and is validated for use in Flow Cyt, ICC, ICC/IF, IHC-P, IP, WB in human, mouse samples.
Anti-CENPF antibody (ab5) specifically detects CENPF (UniProt ID: P49454; Molecular weight: 357kDa) and is sold in 50 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-CENPF antibody (ab5) has high sensitivity and specificity.
Anti-CENPF antibody (ab5) has been cited over 108 times in peer reviewed journals and is trusted by the scientific community.
Anti-CENPF antibody (ab5) has 7 independent reviews from customers.
Target Information
CENPF (Centromere Protein F) is a key protein that participates in cell cycle regulation and chromosome movement during mitosis. It is essential for proper cell division and genomic stability. Abnormal expression of CENPF is associated with several diseases, particularly cancer, where it promotes tumor growth and metastasis. High CENPF levels are observed in cancers like colorectal cancer and are linked to worse clinical outcomes. CENPF is also involved in cardiac development and diseases such as dilated cardiomyopathy.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CENPF also known as centromere protein F or mitosin is a significant kinetochore-associated protein. It has a molecular mass of approximately 367 kDa. Expressed chiefly in the nucleus CENPF displays elevated levels during cell division particularly mitosis. The protein accumulates at the nuclear matrix during the G2 phase of the cell cycle and moves to the kinetochores in mitosis highlighting its role in cell cycle regulation.
Biological function summary
It plays a role in chromosome segregation and spindle attachment during cell division. CENPF associates with various cellular complexes including the nuclear matrix and kinetochores. Its function is critical to maintaining stability and proper chromosome movement during mitosis. This involvement ensures accurate chromosomal alignment and segregation preventing genomic instability in daughter cells.
Pathways
CENPF plays an essential role in the regulation of the mitotic cell cycle and chromosomal dynamics. The protein interfaces with several elements in pathways like the spindle assembly checkpoint. It interacts notably with proteins such as CENP-E and other kinetochore constituents. These interactions safeguard the precise attachment of microtubules to the kinetochores facilitating proper cell cycle progression and division.
Alterations in CENPF expression and function have been linked to cancer and congenital heart defects. Dysregulation of CENPF can lead to chromosomal instability a known hallmark of many cancers. Additionally its interactions with proteins like CENP-E and Bub1 further relate to its involvement in the proper execution of cell cycle checkpoints. Such associations make CENPF a potential target for therapeutic interventions in related disorders.