Abcam, ab50247, Anti-PADI4 / PAD4 antibody

Size: 100µg
Rabbit Polyclonal PADI4 / PAD4 antibody. Suitable for IP, ELISA, WB, ICC/IF and reacts with Human samples. Cited in 24 publications. Immunogen corresponding to Synthetic Peptide within Human PADI4.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IP, WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PADI4. The exact immunogen used to generate this antibody is proprietary information.Q9UM07,
Specificity:This antibody reacts specifically with the 74 kDa PADI4 / PAD4 protein. It shows no cross reactivity with the other isoforms.

Product details:
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 18th July 2017. The following lots is/are still unpurified and still in stock as of 18th July 2017 - GR316454-5, GR316454-6, GR316454-7, GR316454-8, GR316454-9, GR316454-10. Lot numbers other than GR316454-5, GR316454-6, GR316454-7, GR316454-8, GR316454-9, GR316454-10 will be purified. Please note that the dilutions may need to be adjusted accordingly.
Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PADI4 also known as PAD4 PADI4 protein or PAD-4 is an enzyme that plays a mechanical role in post-translational modification. It functions by catalyzing the conversion of arginine residues into citrulline in histones a process known as citrullination or deimination. This enzyme exhibits a molecular mass of approximately 74 kDa and is expressed notably in tissues like bone marrow and sites of inflammation. PADI4 shows high expression in immune cells such as neutrophils which are important for inflammatory responses.
Biological function summary
The PADI4 protein impacts gene expression by modifying chromatin architecture making it part of chromatin remodeling activities. PADI4 is known to associate with chromatin but it does not form large stable complexes. It influences the transcriptional activity of genes by altering histone interactions which can affect immune responses and cellular differentiation. Through these chromatin changes PADI4 plays a notable role in regulating genes associated with immune function and inflammatory responses.
Pathways
PADI4 integrates into the inflammatory response and apoptosis pathways. It influences apoptosis by modulating pro-inflammatory gene expression and participating in the formation of neutrophil extracellular traps (NETs). PADI4 works with other proteins such as histone H3 and histone H4 to influence immune cell pathways. These interactions underline its role in the regulation of protein expressions during inflammation and immune-mediated processes.
PADI4 connects to rheumatoid arthritis (RA) and certain cancers. In rheumatoid arthritis altered activity of PADI4 can lead to the production of autoantibodies against citrullinated proteins which exacerbates inflammation in joints. The enzyme’s dysregulation is also implicated in cancer progression where it may affect tumor growth and metastasis. PADI4’s interaction with other proteins like citrullinated histones highlights its association with pathogenic processes in such diseases.