Abcam, ab50339, Anti-NPHS2 antibody

Size: 50µL
Anti-NPHS2 antibody (ab50339) is a rabbit polyclonal antibody detecting NPHS2 in Western Blot, IHC-P . Suitable for Mouse, Rat . - Over 90 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Podocin aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.Q9NP85

Product details:
What is this antibody validated in?
Anti-NPHS2 antibody (ab50339) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Mouse, Rat samples.
What is the molecular weight of NPHS2?
Anti-NPHS2 (ab50339) specifically detects a band for NPHS2 (UniProt: Q9NP85) at a molecular weight of 42kDa.
Trusted by the scientific community
Anti-NPHS2 (ab50339) was first used in a scientific publication in 2007 and has been cited over 90 times in peer-reviewed journals.
Reviewed by scientists
Anti-NPHS2 (ab50339) has over 10 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab50339 is affinity-purified using the immunizing peptide immobilized on agarose., Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NPHS2 gene encodes the protein known as podocin which plays an important role in the renal system. Podocin is a membrane protein with a molecular mass of approximately 42 kDa. It is largely expressed in the kidney specifically in the podocyte foot processes of the glomerulus. The protein is responsible for the proper functioning of the slit diaphragm a critical component in the kidney's filtration barrier. NPHS2 by maintaining the structural integrity of these cells prevents proteins from spilling into the urine.
Biological function summary
The function of the NPHS2 gene product is significant in the maintenance of the glomerular filtration barrier. Podocin is a part of the slit diaphragm complex interacting closely with other proteins like nephrin. This interaction is required for the formation and stabilization of the slit diaphragm which filters blood plasma in the glomerulus. The precision in its location and function ensures the selective permeability of the filtration barrier.
Pathways
NPHS2 is heavily involved in pathways that regulate kidney function and filtration processes. It is an important component of the slit diaphragm complex pathway. Podocin’s interaction with proteins like nephrin and CD2AP integrates it into signaling pathways important for cytoskeletal dynamics and cell adhesion in podocytes. These interactions play an essential role in facilitating the response of podocytes to mechanical stress and in maintaining the structure of the glomerular filtration barrier.
NPHS2 mutations link directly to steroid-resistant nephrotic syndrome (SRNS). This condition leads to proteinuria and progressive kidney failure. Podocin dysfunction disrupts the integrity of the renal filtration barrier causing this disease. The protein nephrin is another component in SRNS pathophysiology as both are fundamental to maintaining the structure and function of the slit diaphragm. Understanding the relationship between NPHS2 and these proteins can aid in developing targeted therapies for SRNS and related nephropathies.