Abcam, ab50533, Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker

Size: 50µL
Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) is a mouse monoclonal antibody detecting RAB7 in Western Blot . Suitable for Dog, Human, Mouse, Rat . - KO validated for confirmed specificity - Over 120 publications - Trusted since 2007
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:Rab7-117,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Mouse, Rat, Dog, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human RAB7A aa 150-200. The exact immunogen used to generate this antibody is proprietary information.P51149

Product details:
What is this antibody validated in?
Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Dog, Human, Mouse, Rat samples.
What is the molecular weight of RAB7?
Anti-RAB7 [Rab7-117] - Late Endosome Marker (ab50533) specifically detects a band for RAB7 (UniProt: P51149) at a molecular weight of 23kDa.
Trusted by the scientific community
Anti-RAB7 [Rab7-117] - Late Endosome Marker (ab50533) was first used in a scientific publication in 2007 and has been cited over 120 times in peer-reviewed journals.
Reviewed by scientists
Anti-RAB7 [Rab7-117] - Late Endosome Marker (ab50533) has over 25 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RAB7 also known as RAB7A is a small GTPase belonging to the RAS oncogene family. It has a molecular mass of approximately 23 kDa. This protein plays an important role in the regulation of late endocytic trafficking. It is prominently expressed in the cytoplasmic compartments of various cell types and serves as an important late endosome marker. RAB7 controls the maturation of early endosomes into late endosomes and their subsequent fusion with lysosomes therefore influencing degradation pathways.
Biological function summary
RAB7 affects various processes including autophagy cell signaling and pathogen infection response. It often functions as part of the endosomal machinery and forms complexes with other proteins like RAB3GAP1 and RILP. These complexes help mediate vesicle transport and membrane trafficking processes which are essential for the maintenance of cellular homeostasis. RAB7 is significant in ensuring proper lysosomal positioning and function which is critical for cellular metabolism and degradation.
Pathways
RAB7 integrates into the endocytic and autophagic pathways by facilitating the transport between endosomes and lysosomes. It connects with the PI3K/AKT pathway impacting cell proliferation and survival. RAB7 also interacts with the retromer complex influencing the retrieval of receptors from endosomes. Proteins like FYCO1 and Prostaglandin E2 synthase can also cooperate with RAB7 in these pathways further establishing its role in cellular dynamics and signaling.
RAB7 has associations with Charcot-Marie-Tooth disease type 2B and cancer progression. Mutations in the RAB7 gene have been linked to neuropathic conditions leading to impaired motor and sensory function. In the context of cancer dysregulation of RAB7 expression can contribute to increased tumor growth and metastasis. RAB7 interacts with proteins such as MAPK and ARL8B which are involved in these diseases highlighting its importance in pathological conditions.