Abcam, ab5088, Anti-REV1 antibody

Size: 100µg
Goat Polyclonal REV1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human REV1 aa 1200 to C-terminus.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG2c,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human REV1 aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9UBZ9

Product details:
REV1L is the human homolog of the S. cerevisiae mutagenesis protein Rev1. The REV1 protein is conserved from yeast to humans. All REV1 proteins contain a BRCT domain, which is important in protein-protein interactions.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: Tris buffered saline, 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
REV1 also known as REV1 DNA-directed polymerase functions as a DNA polymerase specialized in translesion synthesis. Weighing approximately 125 kDa REV1 facilitates bypass of DNA lesions during replication ensuring continuous DNA synthesis. This protein acts primarily as a deoxycytidyl transferase inserting dCMP opposite damaged nucleotides. REV1 is expressed in various tissues but shows higher expression levels in testis and thymus. It is pivotal in maintaining genome stability under genotoxic stress.
Biological function summary
REV1 operates within the context of DNA repair and a larger polymerase zeta complex working closely with other translesion DNA polymerases. REV1 interacts with other translesion synthesis proteins like POLH POLL and POLK to orchestrate the bypass of different types of DNA damage. These interactions enable REV1 to play a role in cellular responses to DNA damage and contribute to maintaining genome integrity.
Pathways
REV1 integrates into the DNA damage response and translesion synthesis pathways. In these pathways REV1 coordinates with REV3L and REV7 forming part of the polymerase zeta complex to process lesions bypass that regular DNA polymerases cannot handle. Significantly it associates with the Fanconi anemia pathway affecting DNA repair mechanisms under stress. These collaborations help manage replication stress and stabilize cellular division.
REV1 is associated with cancer and immunodeficiency conditions. Abnormalities in its function correlate with faulty translesion synthesis leading to genomic instability implicated in cancer development. Furthermore in conjunction with BRCA1 and BRCA2 issues with REV1 may impact DNA repair fidelity linking it to breast cancer susceptibility. Understanding the role of REV1 in these disorders could lead to therapeutic interventions targeting its pathway interactions and activity.