Abcam, ab51074, Anti-MMP14 antibody [EP1264Y]

Size: 20µL / 100µL / 1mL
Anti-MMP14 antibody [EP1264Y] (ab51074) is a rabbit monoclonal antibody detecting MMP14 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Clone EP1264Y is the most cited clone to MMP14 - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 170 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1264Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IP, Flow Cyt, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
Product Specifications
Anti-MMP14 antibody [EP1264Y] (ab51074) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt, ICC/IF, IHC-P, IP, WB in human, mouse, rat samples.
Anti-MMP14 antibody [EP1264Y] (ab51074) specifically detects MMP14 (UniProt ID: P50281; Molecular weight: 54kDa) and is sold in 100 µL and 1 mL selling sizes.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-MMP14 antibody [EP1264Y] (ab51074) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-MMP14 antibody [EP1264Y] (ab51074) has been confirmed by testing in knockout samples.
Anti-MMP14 antibody [EP1264Y] (ab51074) has been cited over 175 times in peer reviewed journals and is trusted by the scientific community.
Anti-MMP14 antibody [EP1264Y] (ab51074) has 20 independent reviews from customers.
Related Products
Antibody clone EP1264Y is also available pre-conjugated to a variety of labels for your convenience - HRP, Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 488, PE (
ab194242
ab300749
ab302548
ab302582
ab303602
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Matrix metalloproteinase 14 (MMP14) also known as MT1-MMP is a membrane-bound enzyme involved in the breakdown of the extracellular matrix. This protease has a molecular weight of approximately 66 kDa and consists of various structural domains including a catalytic domain and a hinge region. MMP14 is expressed in many tissues including the stromal and cancer cells where it facilitates cell migration and tissue remodeling.
Biological function summary
MMP14 influences extracellular matrix degradation enabling cellular invasion and migration. It exists as a part of macromolecular complexes at the cell surface interacting with other metalloproteinases. This interaction assists in the activation of other MMPs such as pro-MMP2 which further amplify matrix remodeling activities. The hinge region of MMP14 lends flexibility allowing it to effectively engage with substrate proteins.
Pathways
MMP14 significantly affects cell signaling and tissue remodeling pathways. One critical pathway involves the activation of pro-MMP2 which is dependent on MMP14's proteolytic activity. MMP14 also plays a role in the regulation of cell proliferation and apoptosis through its interaction with proteins such as TIMP-2. This relationship between MMP14 and other regulatory proteins emphasizes its functional integration within major biological networks.
High MMP14 levels correlate strongly with cancer progression especially in cancers such as breast cancer and melanoma. The ability of MMP14 to degrade the extracellular matrix facilitates tumor invasion and metastasis. Through its regulatory activities MMP14 connects with other proteins like VEGF influencing angiogenesis within tumor microenvironments. Additionally MMP14 involvement in arthritic conditions highlights its role in joint degradation processes worsening the disease through augmented cartilage breakdown.