Abcam, ab51253, Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]

Size: 100µL / 1mL
Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) is a rabbit monoclonal antibody detecting Alpha-synuclein in Western Blot, IHC-P, IHC-FrFl, ELISA, Dot Blot . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 370 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1536Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:IHC-FrFl, ELISA, Dot, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody only detects alpha synuclein phosphorylated on Ser129. IHC-P: This antibody showed no staining in human hippocampus normal brain and showed staining in Parkinson's brain as expected.Mouse and rat species are recommended based on WB results, we do not guarantee  IHC-P for mouse and rat.

Product details:
Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Dot blot, ELISA, IHC-FrFl, IHC-P, WB in human, mouse, rat samples.
Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) has been cited over 373 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Alpha-synuclein (phospho S129) antibody (Anti-pS129 alpha synuclin antibody/ Anti-SNCA antibody) [EP1536Y] (ab51253) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) has 9 independent reviews from customers.
Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) specifically detects Alpha-synuclein Phospho-S129 (UniProt ID: P37840; Molecular weight: 14kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EP1536Y -
ab209422
Antibody clone EP1536Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 750, PE, Alexa Fluor® 647, Alexa Fluor® 488, Alexa Fluor® 594, Alexa Fluor® 568 (
ab321645
ab314419
ab225294
ab225295
ab311788
ab313069
Alpha-synuclein phosphorylated at serine 129 (pS129; phosphorylated alpha synuclein, SNCA/NACP) is a modified form of the alpha-synuclein protein, which plays a crucial role in the regulation of synaptic vesicle trafficking and neurotransmitter release. This phosphorylation is significantly increased in the brains of patients with Parkinson's disease and other synucleinopathies. The presence of pS129 is associated with the formation of Lewy bodies, a hallmark of Parkinson's disease, and is believed to contribute to the neurotoxic properties of alpha-synuclein, exacerbating disease progression.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha-synuclein also known as SNCA or PARK1 protein is a monomer protein with a molecular weight of approximately 14 kDa. It plays an important role in the synaptic functions of neurons. You usually find this protein expressed in the brain specifically in the presynaptic terminals of neurons. It also appears in other tissues including the heart and muscles though to a lesser degree. In its natural state alpha-synuclein exists as a soluble monomer but under certain conditions it can form insoluble protein filaments. The antibodies 4d6 and ep1536y might be used to study this protein providing tools to investigate its role and behavior in biological processes.
Biological function summary
Alpha-synuclein contributes to the regulation of synaptic vesicle trafficking and release. It interacts with the cell membrane and is involved in the assembly of SNARE complexes important for neurotransmitter release. Although alpha-synuclein generally acts in its monomeric form it may also aggregate and become involved in the assembly of fibrils under pathological conditions impacting normal cellular function. The protein's ability to interact with lipid membranes suggests its possible role in maintaining membrane integrity and dynamics.
Pathways
Alpha-synuclein is integral to neurotransmitter release and synaptic plasticity influencing dopaminergic signaling pathways. This protein interacts with other synaptic proteins like synaptophysin and synaptotagmin linking it to vesicular transport and recycling pathways. It plays a role in regulating dopamine biosynthesis and storage impacting neurological activity. The balance and regulation of these pathways are important for proper neural communication and function.
Alpha-synuclein is strongly associated with neurodegenerative diseases like Parkinson's disease and Lewy body dementia. The accumulation and aggregation of this protein in these disorders form Lewy bodies which are pathological hallmarks. Interactions with proteins like LRRK2 and Parkin also contribute to pathogenesis affecting cellular pathways involved in protein degradation and mitochondrial function. These interactions and aggregations implicate alpha-synuclein as a central figure in the progression and development of these neurological conditions.