Abcam, ab52101, Recombinant human Cleaved Caspase-3 protein (Active)

Size: 100Unit
Recombinant human Cleaved Caspase-3 protein (Active) is a Human Full Length protein, in the 1 to 277 aa range, expressed in Escherichia coli, with >95%, suitable for FuncS.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus His tag C-Terminus,
Applications:FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific activity: ≥ 15,000 units/mgUnit definition: One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37oC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.,
Accession:P42574,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute in water,
Storage buffer:Constituents: PBS, 5% Glycerol (glycerin, glycerine), 1.71% HEPES, 1.71% Sodium chloride, 0.16% EDTA, 0.12% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.1% 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

Product details:
This product is manufactured by BioVision, an Abcam company and was previously called 1083 Caspase-3, human recombinant. 1083-100 is the same size as the 100 unit size of ab52101.
The recombinant active human Caspase 3 spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme.
Cleaved Caspase 3 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. The active Caspase 3 preferentially cleaves Caspase 3 substrates e.g. DEVD-AFC or DEVD-pNA.
MW: large (17 kD) and small (11 kD) subunits.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Cleaved Caspase-3 also known as active caspase 3 plays an important role in the execution phase of cell apoptosis. The caspase 3 molecule has a molecular weight of approximately 32 kDa. It undergoes cleavage into subunits that are important for its activation marking its conversion into the cleaved form. Cleaved caspase is widely expressed in various tissues with a notable presence in the cytoplasm of cells undergoing apoptotic processes. Cleavage of caspase 3 results in the formation of a functional enzyme that participates in the degradation of cellular components during apoptosis.
Biological function summary
Caspase 3 acts as an executioner enzyme in the process of programmed cell death. Its cleavage from the inactive zymogen form into the active form triggers apoptotic pathways ensuring the removal of damaged or unnecessary cells. Cleaved caspase operates within a proteolytic cascade often in conjunction with other caspases such as caspase 9. Once activated caspase 3 targets and cleaves specific cellular substrates contributing to the characteristic morphological changes and DNA fragmentation associated with apoptosis.
Pathways
Cleaved caspase 3 is significant within the intrinsic and extrinsic apoptotic pathways. It acts downstream of initiator caspases such as caspase 9 in the intrinsic pathway where its activation involves mitochondrial signals. In the extrinsic pathway it closely associates with caspase 8 mediating cell death signals from extracellular triggers. These pathways ensure that cleaved caspase 3 fulfills its role as a critical mediator of apoptosis linking upstream death signals to cellular dismantling during the apoptosis process.
Cleaved caspase 3 connects to conditions such as cancer and neurodegenerative diseases. In cancer dysregulation of caspase 3 activity may lead to evasion of apoptosis facilitating tumor progression. Therapeutic approaches aim to restore its apoptotic functions in cancer cells. In neurodegenerative diseases like Alzheimer's disease abnormal activation of cleaved caspase 3 contributes to neuronal loss. It interacts with proteins like amyloid precursor protein promoting the pathological changes associated with this disorder. Understanding these interactions provides insights into potential therapeutic avenues.