Abcam, ab52301, Anti-activated Notch1 antibody

Size: 100µg
Rabbit Polyclonal Notch1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 65 publications. Immunogen corresponding to Synthetic Peptide within Human NOTCH1 aa 1700-1800.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NOTCH1 aa 1700-1800. The exact immunogen used to generate this antibody is proprietary information.P46531,
Specificity:ab52301 detects endogenous levels of fragment of activated Notch 1 resulting from cleavage adjacent to Val1744.

Product details:
ab52301 is cleaved at Val 1744

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab52301 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen., Storage buffer-pH: 7Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Activated Notch1 also known as cleaved Notch1 and Notch 1 NICD is an intracellular domain resulting from Notch1 receptor cleavage. The molecular weight of Notch1 NICD varies but it is generally around 120-150 kDa depending on post-translational modifications. These receptors are present in many cell types including embryonic cells stem cells and various tissue-specific cells. They reside in the cell membrane and become activated upon interaction with specific ligands.
Biological function summary
Activated Notch1 is critical for regulating cell fate decisions including proliferation differentiation and apoptosis. It participates in a multi-protein complex that includes the CSL (CBF1/Su(H)/LAG-1) transcription factor modulating gene expression by binding to DNA. The role of activated Notch1 in maintaining the balance between cell growth and death is important in tissue homeostasis and development.
Pathways
Activated Notch1 plays a central role in the Notch signaling pathway an essential pathway for intercellular communication. This pathway involves several proteins including Delta and Jagged as ligands. It contributes to several critical developmental processes and is connected with the regulation of the NF-kB signaling pathway involved in immune responses and inflammation.
Activated Notch1 is significantly associated with T-cell acute lymphoblastic leukemia (T-ALL) and breast cancer. Abnormal expressions or mutations in Notch1 can lead to unchecked cellular proliferation in these conditions. Notch1 alterations often coexist with disruptions in other signaling proteins such as p53 contributing to disease pathology and presenting potential targets for therapeutic intervention.