Abcam, ab5258, HRP Anti-Interferon alpha 2b antibody [4E10]

Size: 100µL
Mouse Monoclonal Interferon alpha 2 antibody - conjugated to HRP. Suitable for ELISA and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IFNA2.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:4E10,
Isotype:IgG1,
Conjugation:HRP,
Carrier free:No,
Reacts with:Human,
Applications:ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human IFNA2.P01563,
Specificity:Recognizes both human recombinant IFN-a2b and IFN-a2a. Does not react with human recombinant IFN-a5 and IFN-g.

Product details:
Recommended ELISA procedure for human IFN alpha 2b determination: 1. Use 96-well Nunc Maxisorp plates for the assay. Coat microwells with 100 μl/well of antibody 9D3 (
ab9386
) diluted to 10 μg/ml in coating buffer (0.05 M Na-carbonate, pH 9.5). Incubate overnight at +4°C. 2. Aspirate wells and block with 150 μl/well of blocking buffer (2% BSA in PBS). Incubate at RT for 1 h. 3. Aspirate wells and wash 3 times with PBST (0.1% Tween-20 in PBS). 4. Prepare standard and sample dilutions in PBST. Recommended IFN alpha 2b concentrations: from 100 pg/ml to 100 ng/ml. Add 100 μl of each standard and sample into appropriate wells. Incubate at RT for 2 h. 5. Aspirate and wash 5 times with PBST. 6. Dilute detection antibody 4E10-HRP (ab5258) 1:1000 in PBST. Add 100 μl of diluted detection antibody to each well. Incubate at RT for 1 h. 7. Aspirate and wash 10 times with PBST. 8. Add 100 μl of substrate solution (TMB) to each well. Incubate at RT for 10-20 min. 9. Add 50 μl of stop solution (2 N H2SO4) to each well. Read absorbance at 450 nm.
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR308991-1 and GR308991-2 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Interferon alpha 2b also known as IFN-alpha 2b or alpha 2b interferon is a type I interferon protein with an average molecular weight of approximately 19000 Daltons. The protein plays a role in the immune response by exhibiting antiviral antiproliferative and immunomodulatory activities. It is expressed mainly in leukocytes such as lymphocytes macrophages and dendritic cells. Researchers can buy interferon online for laboratory uses or create custom alpha 2b interferon for specific research needs.
Biological function summary
Interferon alpha 2b stimulates the immune system to attack pathogens by enhancing antigen presentation and activating natural killer cells and macrophages. It does not function as part of a complex and it acts independently to initiate an antiviral state in cells and increase the host's defense mechanisms. Its role in regulating cell growth and differentiation adds to its importance in maintaining immune homeostasis.
Pathways
Interferon alpha 2b is involved in the JAK-STAT signaling pathway which induces transcription of interferon-stimulated genes that orchestrate an immune response. Additionally it plays a role in the interferon signaling pathway which contributes to innate immunity. Proteins related to it through these pathways include STAT1 and STAT2 which collaborate with IFN alpha-b protein to transmit signals from extracellular stimuli to the nucleus resulting in gene activation.
Interferon alpha 2b is associated with chronic hepatitis C and certain types of cancer including melanoma. It engages with viral proteins in hepatitis C aiming to clear the virus and reduce liver damage. In cancer the antiproliferative properties of interferon alpha 2b help to inhibit tumor cell growth. In these contexts it connects with proteins such as PKR which helps mediate antiviral and anti-tumor responses.