Abcam, ab52649, Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

Size: 20µL / 100µL / 1mL
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) is a rabbit monoclonal antibody detecting GM130 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for African green monkey, Dog, Human . - Biophysical QC for unrivalled batch-batch consistency - Over 290 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP892Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Dog, Human, African green monkey,
Applications:IP, Flow Cyt (Intra), IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Mouse and rat cell lines pc12, 3t3, raw 264.7 were tested positive in WB. However, brain, kidney, spleen and heart were negative from the two species.

Product details:
Product Specifications
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)  was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB in african green monkey, dog, human samples.
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) specifically detects GM130  (UniProt ID: Q08379; Molecular weight: 113kDa) and is sold in 100 µL and 1 mL selling sizes.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-GM130 antibody [EP892Y] - cis-Golgi Marker  (ab52649) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) has been cited over 294 times in peer reviewed journals and is trusted by the scientific community.
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) has 38 independent reviews from customers.
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Patented technology
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What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
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- Improved sensitivity and specificity
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- Animal-free batch production
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Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GM130 also known as Golgi matrix protein 130 kDa or GM-130 plays an important role in the organization of the Golgi apparatus. The GM130 protein is vital for maintaining the structure of the Golgi serving as a Golgi marker for researchers. Its molecular weight is approximately 130 kDa. You will commonly find GM130 expressed in cells localizing to the cis-Golgi network. As a Golgi marker it serves as an indication of the integrity and positioning of the Golgi apparatus in the cell.
Biological function summary
GM130 serves as a structural component within the Golgi matrix. It interacts with other proteins such as GRASP65 to form a complex which is essential for Golgi stacking and maintenance of Golgi architecture. This interaction helps regulate transport processes inside cells. GM130 proteins help formation of mini-stacks and transitioning of vesicles. In cells GM130 plays a significant role to maintain efficient protein trafficking through the Golgi network.
Pathways
GM130 interacts with proteins like Rab1 during the vesicular transport pathway. It plays a part in the transport of proteins from the endoplasmic reticulum to the Golgi apparatus. Another related pathway involves SNARE proteins whereby GM130 contributes to membrane docking and fusion events. Through these interactions GM130 modulates the fidelity and specificity of molecular cargo transport within the cell.
GM130 has a connection to neurodegenerative diseases such as frontotemporal dementia. Disruption in GM130 proteins affects Golgi structure and function potentially leading to protein trafficking defects associated with the disorder. GM130 also relates to Alzheimer's disease through its interaction with tau protein. Alterations in GM130's function can influence tau pathology contributing to disease progression.