Abcam, ab53084, Anti-Guanylate Cyclase antibody

Size: 100µg
Rabbit Polyclonal GCYB2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human GUCY1B1.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human GUCY1B1. The exact immunogen used to generate this antibody is proprietary information.Q02153

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Guanylate Cyclase also known as GC or GUCY is an enzyme that plays a mechanical role in catalyzing the conversion of guanosine triphosphate (GTP) into cyclic guanosine monophosphate (cGMP) and pyrophosphate. This enzyme exists in two major forms: soluble guanylate cyclase (sGC) and membrane-bound guanylate cyclase (mGC). Soluble guanylate cyclase acts as a receptor for nitric oxide (NO) while membrane-bound guanylate cyclase serves as a receptor for peptide hormones. The molecular mass of soluble guanylate cyclase ranges from about 70 to 82 kDa. Guanylate Cyclase is expressed widely in various tissues including heart brain and vascular smooth muscle handling signaling functions in those areas.
Biological function summary
Guanylate cyclase serves critical signaling functions affecting numerous physiological processes. It is often part of a protein complex and leads to the generation of cGMP a second messenger that influences vascular tone platelet aggregation and neurotransmission. When guanylate cyclase is activated it sets off a cascade of cellular events which modulate these processes by influencing ion channels phosphodiesterases and cGMP-dependent protein kinases. These roles highlight its involvement in maintaining homeostatic functions and facilitating rapid cellular responses to a variety of stimuli.
Pathways
Guanylate cyclase influences significant signaling pathways such as the NO-cGMP pathway and the atrial natriuretic peptide (ANP) pathway. In the NO-cGMP pathway guanylate cyclase interacts prominently with nitric oxide synthases which produce NO to activate sGC leading to cGMP synthesis and smooth muscle relaxation. Similarly in the ANP pathway guanylate cyclase-A (one of the membrane-bound forms) responds to natriuretic peptides by increasing cGMP levels affecting blood volume and pressure. This connectivity further emphasizes its pivotal role in cardiovascular regulation.
Guanylate cyclase holds relevance to disorders like pulmonary hypertension and heart failure. Malfunctions in the NO-cGMP signaling can lead to improper vasodilation contributing to the pathophysiology of pulmonary hypertension. Heart failure can also arise from defects in the ANP pathway that reduce cGMP production and impair heart function. Interaction with proteins such as phosphodiesterase 5 (PDE5) which degrades cGMP becomes significant in these conditions showing how PDE5 inhibitors can target these pathways for therapeutic interventions.