Abcam, ab53126, Anti-Ku70 + Ku80 antibody

Size: 100µg
Rabbit Polyclonal Ku70 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human XRCC6.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human XRCC6. The exact immunogen used to generate this antibody is proprietary information.P12956

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab53126 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ku70 and Ku80 also known collectively as the Ku70/Ku80 complex are proteins that play a vital role in DNA repair. Ku70 and Ku80 are subunits of a heterodimeric protein with Ku70 approximately 70 kDa and Ku80 around 80 kDa in mass. These proteins are typically expressed in the nucleus of eukaryotic cells where they recognize and bind to DNA double-strand break ends. The Ku70/Ku80 complex serves as a scaffold for the assembly of other proteins involved in non-homologous end joining (NHEJ) an important pathway for repairing these DNA breaks.
Biological function summary
Ku70 and Ku80 form a ring-shaped structure that binds tightly around DNA ends facilitating high-fidelity repair processes. They are essential for maintaining genomic stability by ensuring accurate repair of DNA that can otherwise lead to chromosomal aberrations. Ku70/80 operates as part of the DNA-PK complex working alongside DNA-PKcs (DNA-dependent protein kinase catalytic subunit) to promote the NHEJ pathway. This complex orchestrates the repair process by protecting DNA ends and recruiting other essential repair factors.
Pathways
NHEJ and V(D)J recombination are two critical biological pathways where Ku70/Ku80 functions. NHEJ is a major pathway for repairing double-strand breaks especially in the context of DNA damage by radiation or spontaneous genomic instability. V(D)J recombination on the other hand is essential for generating diversity in antigen receptors during immune response. Within these pathways the Ku70/Ku80 complex works closely with DNA ligase IV and XRCC4 to facilitate the final ligation step thereby ensuring effective DNA repair and recombination.
The malfunction of Ku70/Ku80 can lead to severe immunodeficiency disorders and increase the risk of cancer. Deficiencies in the Ku70/Ku80 complex result in a compromised NHEJ repair mechanism leading to increased susceptibility to genomic instability and associated malignancies. Additionally the improper function of Ku70/Ku80 is linked to neurodegenerative diseases due to failed DNA repair also involving other proteins like PARP1 (Poly [ADP-ribose] polymerase 1) highlighting the importance of the complex in maintaining neural health.