Abcam, ab53444, Anti-CD68 antibody [FA-11]

Size: 100µg / 1mg
Anti-CD68 antibody [FA-11] (ab53444) is a rat monoclonal antibody detecting CD68 in Flow Cytometry (Intra), Flow Cytometry, ICC/IF . Suitable for Mouse . - Over 310 publications - Trusted since 2007
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:FA-11,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Mouse,
Applications:ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-CD68 antibody [FA-11] (ab53444) is a rat recombinant monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF in dog, human, mouse, rat samples.
Anti-CD68 antibody [FA-11] (ab53444) has been cited over 312 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-CD68 antibody [FA-11] (ab53444) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CD68 antibody [FA-11] (ab53444) has 24 independent reviews from customers.
Anti-CD68 antibody [FA-11] (Anti-Macrosialin antibody) (ab53444) specifically detects CD68 (UniProt ID: P34810; Molecular weight: 35kDa) and is sold in 100 µg and 1 mg selling sizes.
Conjugation-ready, carrier free format available for antibody clone FA-11 -
ab237968
Antibody clone FA-11 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 405, Alexa Fluor® 488, Alexa Fluor® 647, HRP, PE, PerCP, APC (
ab199571
ab201844
ab201845
ab216526
ab216701
ab220509
ab221251
CD68 is a transmembrane glycoprotein predominantly expressed on macrophages, playing a crucial role in the immune system. It is involved in phagocytosis and antigen presentation, making it essential for immune responses. CD68 is expressed in various macrophage subtypes, including M1 (pro-inflammatory) and M2 (anti-inflammatory), contributing to both inflammation and tissue repair. In the central nervous system, CD68 is a marker for microglia, the brain's immune cells, and is involved in clearing cellular debris and modulating inflammatory responses. Its expression levels can influence the progression of neurodegenerative diseases and other immunological conditions.
Although some customers have had success with this antibody in IHC-P, we are unable to obtain positive results in this application and so cannot recommend it for IHC-P. We batch test the antibody in IHC-Fr.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant., Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.
Biological function summary
CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.
Pathways
CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.
CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.