Abcam, ab53449, Biotin Anti-Polyethylene glycol antibody [PEG-B-47b]

Size: 100µg
Anti-Polyethylene glycol antibody [PEG-B-47b] - Biotin conjugated (ab53449) is a rabbit recombinant monoclonal antibody detecting Polyethylene glycol in Western Blot, IHC-P, ELISA . - Biophysical QC for unrivalled batch-batch consistency - Over 10 publications - Trusted since 2007
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:PEG-B-47b,
Isotype:IgG,
Conjugation:Biotin,
Carrier free:No,
Applications:WB, IHC-P, ELISA, sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:ab53449 does not cross react with Tween20.

Product details:
Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)  was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ELISA, IHC-P, WB, sELISA.
Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) has been cited over 15 times in peer reviewed journals and is trusted by the scientific community.
Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) is sold in a 100 ug selling size.
Conjugation-ready, carrier free format available for antibody clone (PEG-B-47b) -
ab271853
The smallest PEG molecule which our Polyethylene glycol antibody (ab53449) can detect is PEG750. Based on customer feedback, the largest PEG molecule detected is PEG 40K. Additionally, ab53449 detects both linear and branched PEG based on our in-house ELISA testing.
Tween 20 usage:
Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. Our anti-mPEG antibody [PEG-B-47b] reacts to the methoxy group of Polyethylene glycol (PEG). There is no methoxy group in Tween 20, which should not interfere with the function of this antibody.
If you have any questions on our PEG products - please visit our
Polyethylene glycol (PEG) FAQs
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle