Abcam, ab5482, Anti-RAC1 + Cdc42 (phospho S71) antibody

Size: 50µL
Rabbit Polyclonal RAC1 phospho S71 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human RAC1 phospho S71.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human RAC1 phospho S71. The exact immunogen used to generate this antibody is proprietary information.P63000,
Specificity:Cdc 42 [pS71] (100% homologous) and Rho A/B/C [pS73] (92% homologous) are expected to react.

Product details:
RAC, Cdc 42 and Rho A, B, and C are members of a small RhoGTPase family that bind and hydrolyze GTP. GTP bound RAC 1 and cdc 42 play a pivotal role in controlling cell shape, adhesion, growth and transformation. Active Rac 1 is implicated in regulating serum response element (SRE), NFAT 1 and nuclear factor kappa B (NF kappa B) transcription activities. Activated RAC 1 and Cdc 42 bind and activate PAK 1, which in turn activates key downstream signaling proteins including MEKK 1 and JNK. RAC 1 and Cdc 42 are phosphorylated on serine 71, a putative Akt site located between the protein binding domain and GTP binding domain. Phosphorylation of RAC 1 on serine 71 regulates its GTP binding and GTPase activity.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated RAC 1. The final product is generated by affinity chromatography using a RAC 1 derived peptide that is phosphorylated at serine 71., Storage buffer-pH: 7.3Preservative: 0.05% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RAC1 and Cdc42 are small Rho GTPases involved in regulating various cellular processes related to the cytoskeleton. RAC1 also known as Ras-related C3 botulinum toxin substrate 1 has a molecular mass of approximately 21 kDa. These proteins are found in a wide range of tissues displaying high expression levels in places like the brain and immune cells. They function as molecular switches cycling between an active GTP-bound state and an inactive GDP-bound state facilitating signal transduction for numerous cellular responses.
Biological function summary
RAC1 and Cdc42 critically influence actin cytoskeleton dynamics and cell morphology. They are part of the Rho family small GTPase interactive with downstream effectors to control cell shape movement and division. These proteins play significant roles in membrane trafficking cell cycle progression and apoptosis. RAC1 along with Cdc42 forms part of a complex network that is essential for functional cytoskeleton reorganization important in neuronal activity and immune cell functioning.
Pathways
RAC1 and Cdc42 mediate signal transduction in the PI3K/AKT pathway and the Wnt signaling pathway. In the PI3K/AKT pathway these proteins interact with other components like PAK proteins which serve as essential mediators for survival and growth signals. In the Wnt pathway RAC1 and Cdc42 influence gene transcription helping control cellular proliferation and differentiation. Their roles in these pathways show their importance in maintaining cellular homeostasis.
RAC1 and Cdc42 connect to cancer and neurological disorders. Alterations in their activity can contribute to tumor growth and metastasis as their dysregulation affects cell migration and invasion. RAC1 is especially noted for its role in various cancer types. Additionally their imbalance may lead to neurological disorders due to their effect on nerve growth and branching. RAC1 and Cdc42's interactions with proteins like RhoA and β-catenin underline their significance in pathophysiological conditions making them critical targets for therapeutic exploration.