Abcam, ab57491, Anti-Exportin-5 antibody [2C5-1B3]

Size: 50µg
Mouse Monoclonal Exportin-5 antibody. Suitable for IHC-P, IP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human XPO5.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2C5-1B3,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WB, IP, IHC-P, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human XPO5.Q9HAV4

Product details:
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Purification notes-Purified from TCS., Storage buffer-pH: 7.4, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Exportin-5 also known as Exp5 and eXportin-5 serves a mechanical role in the cellular export of specific RNA molecules from the nucleus to the cytoplasm. This protein belongs to the karyopherin family and has an approximate molecular mass of about 130 kDa. Exportin-5 primarily expresses in cell types where RNA processing and regulation are actively important though specific distribution patterns vary across different tissues.
Biological function summary
Exportin-5 functions by specifically recognizing and binding to pre-microRNA and small nuclear RNA facilitating their transport out of the nucleus. It operates as a part of a complex with RanGTP which ensures that the RNA cargo is correctly recognized and loaded for transport. This export process is essential for the subsequent RNA maturation and ultimately influences gene expression regulation.
Pathways
While Exportin-5 plays a pivotal role in the RNA transport pathway it is also intricately linked to the microRNA biogenesis pathway. Within these pathways Exportin-5 works closely with RanGTP to mediate the nuclear export of pre-microRNAs setting the stage for their maturation into functional microRNAs in the cytoplasm. It also relates to proteins involved in the RNA-induced silencing complex (RISC) assembly as mature microRNAs are integral parts of RISC.
Exportin-5 appears to be linked to tumorigenesis and certain viral infections. Abnormalities in its function may disrupt microRNA export which can lead to dysregulation of gene expression and contribute to cancer development. Furthermore associations with proteins such as Dicer and RISC-related factors highlight its role in antiviral responses where the microRNA pathway can be modulated by viral mechanisms to evade host defenses.