Abcam, ab5828, Anti-Y14 antibody [4C4]

Size: 100µL
Mouse Monoclonal Y14 antibody. Suitable for IHC-P, ICC, Flow Cyt, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human RBM8A.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:4C4,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Human,
Applications:ICC, WB, IHC-P, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Human RBM8A.Q9Y5S9

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purified from tissue culture supernatant., Storage buffer-Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Y14 also known as RBM8A is a protein integral to the exon junction complex (EJC) with an approximate mass of 18 kDa. It localizes mainly in the nucleus of cells. Y14 works with other proteins such as MAGOH serving a role in post-splicing mRNA regulation. Across various tissues Y14 shows expression but is particularly notable in tissues with high levels of mRNA processing like the brain and testis.
Biological function summary
Y14 functions in mRNA surveillance and quality control mechanisms. It forms part of the exon junction complex a multi-protein structure deposited on mRNA after splicing occurs. The component acts importantly in nonsense-mediated mRNA decay (NMD) a process that ensures defective mRNAs do not translate into dysfunctional proteins. Y14 contributes significantly to mRNA export from the nucleus binding to spliced mRNA and guiding them towards translation sites in the cytoplasm.
Pathways
Y14 plays a significant role in the nonsense-mediated mRNA decay pathway working in tandem with proteins such as UPF1 and UPF3. This pathway helps identify and degrade mRNA transcripts with premature stop codons therefore protecting cells from expressing truncated proteins that may disrupt normal cellular functions. Moreover Y14 is linked to the mRNA export pathway ensuring that only properly spliced messages reach the ribosome for translation.
Y14 relevance appears in relation to neurodevelopmental disorders and certain cancers. Mutations or dysregulation of Y14 might contribute to conditions such as intellectual disability due to its involvement in mRNA surveillance that affects neuronal development. Additionally aberrant expression of Y14 has connections to breast cancer where it interacts with proteins involved in altered signaling pathways impacting cancer progression and metastasis.