Abcam, ab58755, Anti-Trichohyalin antibody [AE15]

Size: 100µL
Mouse Monoclonal Trichohyalin antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Human, Mouse samples. Cited in 17 publications. Immunogen corresponding to Tissue preparation containing TCHH protein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:AE15,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:IHC-P, WB, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Tissue preparation containing TCHH protein. The exact immunogen used to generate this antibody is proprietary information.Q07283,
Specificity:This antibody recognises a 220K trichohyalin granular protein, a marker for hair follicular differentiation.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.1% Sodium azide Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Trichohyalin (THH) acts mechanically as a structural protein that plays an important role in hair and skin cell cornification. Expressed in the inner root sheath of hair follicles and in the granular layer of the epidermis it contributes to cell envelope formation. This protein with a molecular mass of approximately 250 kDa is also known as trichohyalin-1. THH is rich in glutamine and lysine which allows it to bind to other proteins and form a stable matrix structure in hair and skin.
Biological function summary
Trichohyalin contributes to the development and maintenance of hair follicle integrity and skin barrier function. As part of the keratinocyte differentiation process it interacts with various keratins and contributes to cell envelope formation by reinforcing cell membranes through cross-linking. The protein forms a part of the cornified cell envelope complex where it binds with filaggrin and loricrin aiding in cellular structural support and resistance to mechanical stress.
Pathways
Trichohyalin integrates into the cellular pathways involved in keratinocyte differentiation and cornification. It participates closely in the epidermal differentiation complex a critical pathway for skin barrier function. Trichohyalin works alongside proteins such as involucrin and filaggrin to facilitate these processes ensuring the maintenance of skin homeostasis and protective barrier formation.
The altered expression or malfunction of trichohyalin links to disorders like ichthyosis vulgaris and hair shaft abnormalities. These conditions involve aberrations in the epidermal differentiation pathway. Mutations in trichohyalin can affect hair texture and fragility often seen in conjunction with other keratin-related abnormalities. Researchers observe that defective trichohyalin also relates to structural skin anomalies associated with the malfunction of proteins like loricrin and involucrin.