Abcam, ab59551, Anti-Eph receptor A2 antibody [Ka-5H5]

Size: 100µg
Mouse Monoclonal Eph receptor A2 antibody. Carrier free. Suitable for Flow Cyt, ICC/IF and reacts with Transfected cell line - Human, Human samples. Cited in 1 publication. Immunogen corresponding to Nucleic Acid cDNA encoding human EphA2 within Human EPHA2.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:Ka-5H5,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Nucleic Acid cDNA encoding human EphA2 within Human EPHA2. The exact immunogen used to generate this antibody is proprietary information.P29317,
Specificity:ab59551 recognises the complete native EphA2 protein expressed on transfected mammalian cells.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.2Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Eph receptor A2 also known as EphA2 or A2 receptor is a type of protein that functions as a receptor tyrosine kinase. It has an approximate mass of 130 kDa. Researchers find this receptor widely expressed in various tissues including epithelial cells and some neuronal populations. EphA2 is involved in cell positioning movement and organization by interacting with ephrin ligands notably ephrin A1. The receptor-ligand interaction triggers bidirectional signaling that affects cell structure and function.
Biological function summary
Eph receptor A2 participates in important cellular processes such as angiogenesis cell proliferation and migration. It often functions as part of larger signaling networks or complexes that include other Eph receptors and ephrin ligands. These interactions help regulate cell adhesion and detachment which are important for embryonic development and tissue organization. EphA2 studies often use fluorescent labeling strategies such as Alexa 588 to track its expression and activity in cells.
Pathways
Eph receptor A2 integrates into the PI3K/AKT and MAPK signaling pathways which are important for cell survival growth and differentiation. It interacts with proteins like Ras and ERK within these pathways to modulate their functions. EphA2 signaling influences cytoskeletal dynamics and morphogenesis making it an important component in maintaining tissue architecture. Research also indicates a connection between EphA2 and the APC protein highlighting its role in the Wnt signaling pathway.
The Eph receptor A2 shows significant involvement in cancer and inflammatory conditions. Overexpression or dysregulation of EphA2 occurs frequently in various cancers including breast and prostate cancer correlating with increased tumor aggressiveness and metastasis. The receptor also interacts with other proteins such as APC which links to tumorigenesis and cancer progression. In immunology EphA2 plays a part in chronic inflammatory diseases by modulating immune cell migration and vascular inflammation. Understanding these connections provides insights for potential therapeutic targets using molecular interventions like A2 antibodies and APC-based strategies.