Abcam, ab59697, Anti-Macrophages + Monocytes antibody [ER-HR3]

Size: 50µg
Rat Monoclonal Mouse Macrophage antibody. Suitable for Flow Cyt, IHC-P, IHC-Fr and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing Mouse Macrophage protein.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:ER-HR3,
Isotype:IgG2c,
Carrier free:No,
Reacts with:Mouse,
Applications:IHC-P, Flow Cyt, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is reactive to the majority of mouse monocytes and a subset of mature resident macrophages, especially those located in hemopoietic organs. Whereas all other leukocytes are negative, up to 70% of circulating monocytes stain positive with this antibody. About 30% of bone marrow cells express the antigen. Other positive macrophages can be found in the splenic red pulp, in the mesentric lymphoid paracortex, interfollicular areas of Peyer's patches and bone marrow. Few positive cells are observed in the thymic cortex and the connective tissues of the gastro-intestinal tract, the dermis and the renal medulla. Epidermal Langerhans cells also express the antigen.

Product details:
This antibody can be used to identify and localize a very distinct macrophage subpopulation found in various mouse organs and its distribution pattern is unique compared to that from other macrophage-specific antibodies. The antibody is especially suitable for ontogenic studies because ER-HR3 positive macrophages are closely related to hemopoietic islands, especially at erythropoietic sites.
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Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified antibody in 0.2µm filtered solution., Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle